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Introduction. Blood borne infections have a decisive role in the evolution of the liver transplant patient. Detecting these infections remains an absolute priority. Objectives. To compare the ability of two automated microbiological screening systems (VersaTREK versus BACTEC 9050) in detecting the bacterial and fungal etiology of hemocultures in the curable-phases of infection in liver transplant patients, in order to apply the timely and guided antibiotic and antifungal treatment. Material and method. The microbiological study was performed on 60 liver transplant patients (38 men and 22 women) at the "Dan Setlacec" General Surgery and Liver Transplantation Centre from Fundeni Clinical Institute. This study was performed from February to November 2013, in patients with at least 2-3 episodes of transient bacteremia and / or in postoperative sepsis, with 1 to 30 CFU / ml blood the limit of detection. 480 blood cultures from both systems were collected, 240 aerobic and 240 anaerobic. VersaTREK bottles (Redox 1/40 ml, Redox 2/40 ml) were compared with aerobic and anaerobic standard BACTEC/F. By comparing these systems, we detected isolated bacteria and fungi from positive blood cultures. Results. The time to growth detection from the moment of incubation was 18 hours for all organisms on the VersaTREK system, with detection sensitivity of fewer than 20 CFU / ml, as compared to the BACTEC system where growth was detected at 24 hours. We isolated a percent of 30% Gramnegative bacilli (non-fermenters and fermenters), 21.6% MRSA and 6.6% Candida albicans. False positive results were detected - 16.6% for VersaTREK and 25% on BACTEC. Contaminations were particularly present when blood was drawn from an indwelling catheter and less often when a percutaneous venipuncture was performed. For the VersaTREK system, an inoculum of 0.1 ml yielded detection and isolation of germs after only 3 hours after incubation. Conclusions. Both systems were effective in detection of bacteria and fungi in liver transplant patients, but in the case of the VersaTREK system germs were detected and isolated from 0.1 ml inoculum at >3 hours from incubation [ABSTRACT FROM AUTHOR]

Introduction. Blood borne infections have a decisive role in the evolution of the liver transplant patient. Detecting these infections remains an absolute priority. Objectives. To compare the ability of two automated microbiological screening systems (VersaTREK versus BACTEC 9050) in detecting the bacterial and fungal etiology of hemocultures in the curable-phases of infection in liver transplant patients, in order to apply the timely and guided antibiotic and antifungal treatment. Material and method. The microbiological study was performed on 60 liver transplant patients (38 men and 22 women) at the "Dan Setlacec" General Surgery and Liver Transplantation Centre from Fundeni Clinical Institute. This study was performed from February to November 2013, in patients with at least 2-3 episodes of transient bacteremia and / or in postoperative sepsis, with 1 to 30 CFU / ml blood the limit of detection. 480 blood cultures from both systems were collected, 240 aerobic and 240 anaerobic. VersaTREK bottles (Redox 1/40 ml, Redox 2/40 ml) were compared with aerobic and anaerobic standard BACTEC/F. By comparing these systems, we detected isolated bacteria and fungi from positive blood cultures. Results. The time to growth detection from the moment of incubation was 18 hours for all organisms on the VersaTREK system, with detection sensitivity of fewer than 20 CFU / ml, as compared to the BACTEC system where growth was detected at 24 hours. We isolated a percent of 30% Gramnegative bacilli (non-fermenters and fermenters), 21.6% MRSA and 6.6% Candida albicans. False positive results were detected - 16.6% for VersaTREK and 25% on BACTEC. Contaminations were particularly present when blood was drawn from an indwelling catheter and less often when a percutaneous venipuncture was performed. For the VersaTREK system, an inoculum of 0.1 ml yielded detection and isolation of germs after only 3 hours after incubation. Conclusions. Both systems were effective in detection of bacteria and fungi in liver transplant patients, but in the case of the VersaTREK system germs were detected and isolated from 0.1 ml inoculum at >3 hours from incubation [ABSTRACT FROM AUTHOR]