1. Bacterial biofilm in adenoids of children with chronic otitis media. Part I: a case control study of prevalence of biofilms in adenoids, risk factors and middle ear biofilms.
- Author
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Kania R, Vironneau P, Dang H, Bercot B, Cambau E, Verillaud B, Camous D, Lamers G, Herman P, Vicaut E, Tessier N, and Van Den Abbeele T
- Subjects
- Adenoidectomy, Case-Control Studies, Child, Child, Preschool, Chronic Disease, Humans, Infant, Otitis Media surgery, Prospective Studies, Risk Factors, Adenoids microbiology, Biofilms, Ear, Middle microbiology, Otitis Media microbiology
- Abstract
Background: Biofilms are communities of bacteria embedded in a self-produced glycocalyx matrix. Adenoids have been shown to harbor bacterial biofilms. Aim/objectives: To compare the prevalence of biofilms in adenoid of children with chronic otitis media (COM) (group1) versus a control group without any COM (group 2) having adenoids removed because of hypertrophy., Material and Methods: One hundred and three children were prospectively enrolled in this case-control study, group 1 (n = 52) and group 2 (n = 51). The main outcome measurement was the prevalence of biofilm in adenoidectomy specimens analyzed using confocal laser scanning microscopy. Children in group 1 who had middle ear (ME) effusion and requiring the insertion of a tympanostomy tube underwent biopsy of the ME mucosa and effusion sampling., Results: Biofilms were found in adenoids' specimens of both groups and in the ME biopsy and effusion. The biofilm prevalence in adenoids was 63.5% (33/52) in group 1 and 47.1% (24/51) in group 2. Day nursery and previous antibiotics intake were significantly more frequent in group 1 than in group 2., Conclusions and Significance: This case-control study demonstrates that adenoid tissue in children with COM contains more mucosal biofilms than adenoid tissue removed for hypertrophy. Biofilm was seen in ME biopsies and effusion.
- Published
- 2019
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