1. Dose-dependent effects of ouabain on spiral ganglion neurons and Schwann cells in mouse cochlea
- Author
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Hong-Xia Guan, Tao Zhou, Yang Jiang, Qing-Quan Hua, Bokui Xiao, Hua Liao, Kun Yang, and Zhi-Jian Zhang
- Subjects
0301 basic medicine ,Hearing Loss, Sensorineural ,Auditory neuropathy ,Biology ,Ouabain ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Hair Cells, Auditory ,otorhinolaryngologic diseases ,medicine ,Animals ,Hearing Loss, Central ,Enzyme Inhibitors ,Spiral ganglion ,Cochlea ,Round window ,Dose-Response Relationship, Drug ,General Medicine ,Anatomy ,medicine.disease ,Cell biology ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Otorhinolaryngology ,Immunohistochemistry ,Female ,Sensorineural hearing loss ,Schwann Cells ,sense organs ,Brainstem ,Spiral Ganglion ,030217 neurology & neurosurgery ,medicine.drug - Abstract
This study aimed in fully investigating the toxicities of ouabain to mouse cochlea and the related cellular environment, and providing an optimal animal model system for cell transplantation in the treatment of auditory neuropathy (AN) and sensorineural hearing loss (SNHL).Different dosages of ouabain were applied to mouse round window. The auditory brainstem responses and distortion product otoacoustic emissions were used to evaluate the cochlear function. The immunohistochemical staining and cochlea surface preparation were performed to detect the spiral ganglion neurons (SGNs), Schwann cells and hair cells.Ouabain at the dosages of 0.5 mM, 1 mM and 3 mM selectively and permanently destroyed SGNs and their functions, while leaving the hair cells relatively intact. Ouabain at 3 mM resulted in the most severe SGNs loss and induced significant loss of Schwann cells started as early as 7 days and with further damages at 14 and 30 days after ouabain exposure.The application of ouabain to mouse round window induces damages of SGNs and Schwann cells in a dose- and time-dependent manner, this study established a reliable and accurate animal model system of AN and SNHL.
- Published
- 2017