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Start Over Descriptor "p16 Immunohistochemistry" Journal acta oto-laryngologica
3 results on '"p16 Immunohistochemistry"'

1. Human papillomavirus and p16 protein expression as prognostic biomarkers in mobile tongue cancer.

Author

Minami, Kazuhiko, Kogashiwa, Yasunao, Ebihara, Yasuhiro, Nakahira, Mitsuhiko, Sugasawa, Masashi, Fujino, Takashi, and Yasuda, Masanori

Subjects
*PAPILLOMAVIRUS disease diagnosis, *SMOKING, *BIOMARKERS, *COLLECTION & preservation of biological specimens, *CANCER patients, *CHI-squared test, *STATISTICAL correlation, *DNA, *ALCOHOL drinking, *GENE expression, *GENES, *HEAD tumors, *IMMUNOHISTOCHEMISTRY, *MULTIVARIATE analysis, *NECK tumors, *ONCOGENES, *PAPILLOMAVIRUS diseases, *PAPILLOMAVIRUSES, *POLYMERASE chain reaction, *PROTEINS, *RESEARCH funding, *RNA, *SURVIVAL, *TUMOR classification, *DISEASE prevalence, *PROPORTIONAL hazards models, *RETROSPECTIVE studies, *DATA analysis software, *KAPLAN-Meier estimator, *DISEASE complications, *DIAGNOSIS, TONGUE tumors
Abstract

Objectives: The objectives were to determine the prevalence of human papillomavirus (HPV) in mobile tongue cancer (MTC) and evaluate associations and survival. Materials and methods: Patients who underwent surgical resection as primary treatment for MTC (n = 127) were retrospectively evaluated. Formalin-fixed paraffin-embedded (FFPE) specimens were assessed for p16 and p53 by immunohistochemistry; for HPV DNA by nested multiplex polymerase chain reaction (PCR) using two pairs of consensus primers (MY09–MY11 and GP5+–GP6+); and for E6 and E7 oncogenes from 13 high-risk HPV types (16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66, and 68) by real-time reverse transcription PCR (RT-PCR). Results: There were 18 (14.2%) p16-positive, 45 (35.4%) p53-positive, 9 (7.1%) HPV DNA-positive, and 7 (5.5%) E6 and/or E7 mRNA-positive tumors, but the correlation of all pairs was poor. There was no demographic or histopathologic association with HPV status. Cause-specific survival was significantly better with p16-positive than with p16-negative tumors (p =.037). Conclusions: The prevalence of HPV and p16 positivity was relatively low and p16 status was a poor surrogate marker for HPV status. The results showed the importance of p16 expression in prognosticating mobile tongue cancer. [ABSTRACT FROM AUTHOR]

Published
2017
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2. A clinical analysis of sinonasal squamous cell carcinoma: a comparison of de novo squamous cell carcinoma and squamous cell carcinoma arising from inverted papilloma

Author

Ryuji Yasumatsu, Takahiro Hongo, Ryutaro Uchi, Masahiko Taura, Rina Jiromaru, Takashi Nakagawa, Mioko Matsuo, and Takahiro Wakasaki

Subjects
Human papilloma virus, Pathology, medicine.medical_specialty, P16 immunohistochemistry, Clinical pathology, business.industry, Inverted papilloma, General Medicine, Aggressive disease, medicine.disease, stomatognathic diseases, Rare tumor, Otorhinolaryngology, Medicine, Basal cell, business, neoplasms
Abstract

Background: Sinonasal squamous cell carcinoma (SCC) is a rare tumor arising either de novo or in association with inverted papillomas (IPs).Objectives: The aim of this study was to investigate and compare the oncological features and prognosis of patients with sinonasal SCCs based on their etiology.Material and methods: The medical records of 117 patients who had been diagnosed with de novo SCC or those arising from IP (IP-SCC) were retrospectively reviewed. In situ hybridization analyses to detect HPV 16/18DNA and p16 immunohistochemistry were also performed in 10 cases with IP-SCC.Results: The three-year disease-specific survival (DSS) rate was higher in cases with T1, 2 and 3 than in cases with T4 in both tumor groups. T4 cases with de novo SCC had a better DSS than those with IP-SCCs. HPV16/18 was not detected in any of the 10 IP-SCCs.Conclusions and significance: T4 cases with de novo SCC tended to have a better DSS than those with IP-SCC. Since some T4 patients with IP-SCC were found to have a highly aggressive disease, careful treatment planning should be performed. High-risk HPV may not play a vital role in the carcinomatous transformation of most IP-SCC cases.

Published
2020

3. p16 immunohistochemistry alone is a better prognosticator in tonsil cancer than human papillomavirus in situ hybridization with or without p16 immunohistochemistry

Author

Soon Yuhl Nam, Sang Wook Lee, Jiyoun Kim, Jonh Lyel Roh, Dok Hyun Yoon, Kyung Ja Cho, Si Yeol Song, Seung Ho Choi, Sung-Bae Kim, Jeong Hwan Back, Kwonoh Park, Miji Lee, Myung Woul Han, and Sang Yoon Kim

Subjects
Oncology, Adult, Male, medicine.medical_specialty, Pathology, Alcohol Drinking, Tonsillar Neoplasms, In situ hybridization, Immunoenzyme Techniques, Internal medicine, Republic of Korea, medicine, Tonsil cancer, Humans, Human papillomavirus, Cyclin-Dependent Kinase Inhibitor p16, In Situ Hybridization, Aged, Neoplasm Staging, Proportional Hazards Models, P16 immunohistochemistry, Human papillomavirus 16, Tissue microarray, business.industry, Papillomavirus Infections, Smoking, virus diseases, General Medicine, Middle Aged, medicine.disease, Prognosis, Head and neck squamous-cell carcinoma, Combined Modality Therapy, Neoplasm Proteins, Survival Rate, Otorhinolaryngology, Tonsillar Squamous Cell Carcinoma, Tissue Array Analysis, Lymphatic Metastasis, Carcinoma, Squamous Cell, Immunohistochemistry, Female, business
Abstract

p16 immunohistochemistry (IHC) status correlated with less exposure to smoking and/or alcohol in Korean patients with locally advanced tonsillar squamous cell carcinoma (TSCC), and was an independent prognostic factor for survival.TSCC is more likely to be human papillomavirus (HPV)-positive than other head and neck squamous cell carcinoma (HNSCC) subtypes. The objective of this study was to ascertain the HPV status of TSCC in Korean patients and to determine its relationship with clinical parameters and prognosis.The locally advanced TSCCs of 79 patients who were treated between 2000 and 2008 were tested by p16 IHC and HPV in situ hybridization (ISH) with a tissue microarray.Sixty-three patients (80%) were positive for p16 IHC, while 54 (68%) were positive by HPV ISH. p16 IHC status correlated significantly with lower exposure to smoking and alcohol (p0.05) but did not correlate with T and N stage classification, histological differentiation, age, or gender. The p16-positive group had a significantly higher 5-year overall survival rate in comparison with the p16-negative group (78% vs 63%, hazard ratio (HR) = 0.347, 95% CI = 0.14, 95% Cp = 0.025). p16 IHC was a favorable independent prognostic factor for overall survival, even after adjustment for age and T stage (HR = 0.283, 95% CI = 0.103, 95% p = 0.015).

Published
2012

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