1. Entamoeba histolytica L220 induces the in vitro activation of macrophages and neutrophils and is modulated by neurotransmitters.
- Author
-
Villalobos-Gómez FDR, García-Lorenzana M, Escobedo G, Talamás-Rohana P, Salinas-Gutiérrez R, Hernández-Ramírez VI, Sánchez-Alemán E, Campos-Esparza MDR, Muñoz-Ortega MH, and Ventura-Juárez J
- Subjects
- Adolescent, Cytokines drug effects, Cytokines immunology, Entamoeba histolytica chemistry, Epinephrine pharmacology, Humans, Lectins chemistry, Lectins immunology, Lectins pharmacology, Macrophages microbiology, Macrophages physiology, Neurotransmitter Agents immunology, Neutrophils microbiology, Neutrophils physiology, Nicotine pharmacology, Protozoan Proteins chemistry, Protozoan Proteins immunology, Vecuronium Bromide pharmacology, Young Adult, Entamoeba histolytica immunology, Macrophage Activation drug effects, Macrophages drug effects, Neurotransmitter Agents pharmacology, Neutrophil Activation drug effects, Neutrophils drug effects, Protozoan Proteins pharmacology
- Abstract
The neuroimmunoregulation of inflammation has been well characterized. Entamoeba histolytica provokes an inflammatory response in the host in which macrophages and neutrophils are the first line of defense. The aim of this study was to analyze the effect of the 220 kDa lectin of Entamoeba histolytica on stimulation of human macrophages and neutrophils, especially the secretion of cytokines and the relation of these to neurotransmitters. Human cells were interacted with L220, epinephrine, nicotine, esmolol and vecuronium bromide. The concentrations of IL-1β, IFN-γ, TNF-α and IL-10 were determined by ELISA at, 4 h of interaction. L220 has a cytokine stimulating function of macrophages and neutrophils for secretion of IL-1β, and IL-10 only by macrophages, which was modulated by the effect of vecuronium on cholinergic receptors in this immune cells.
- Published
- 2018
- Full Text
- View/download PDF