1. Molecular characterization of SCARECROW (CsSCR) gene expressed during somatic embryo development and in root of cucumber (Cucumis sativus L.)
- Author
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Anna Pietraszewska-Bogiel, Marcin Filipecki, Stefan Malepszy, Anita Wiśniewska, Norikazu Tagashira, Sabina Zuzga, and Barbara Łotocka
- Subjects
Genetics ,Reporter gene ,Somatic embryogenesis ,Somatic cell ,Physiology ,Plant Science ,Biology ,biology.organism_classification ,Cell biology ,Gene expression ,Arabidopsis thaliana ,Endodermis ,Gene ,Cucumis ,Agronomy and Crop Science - Abstract
Somatic embryogenesis (SE) in plants can be used as a model for studying genes engaged in the embryogenic transition of somatic cells. The CsSCARECROW (CsSCR) gene was previously identified among a panel of genes upregulated after the induction of SE in cucumber (Cucumis sativus). The putative CsSCR protein contains conserved GRAS family domains and is extremely similar to AtSCR from Arabidopsis thaliana. SCR proteins are transcription factors involved in root radial patterning and are required for main- tenance of the quiescent centre and differentiation of the endodermis. In comparison with other GRAS proteins from cucumber, phylogenetic analyses showed that CsSCR belongs to the SCR cluster. Increased CsSCR transcript accumulation was detected in somatic embryos and roots. Southern blot analysis and screening of the draft version of the cucumber genome confirmed the lack of close homologues in this spe- cies. CsSCR transcripts were localized by in situ hybridization in undifferentiated cells in the globular and heart stages of somatic embryogenesis, and in the endodermis of torpedo and cotyledonary stage somatic embryos, and developing primary and lateral roots. This localization was supported by the pattern of reporter gene activity driven by the CsSCR promoter in transgenic cucumber organs. These results suggest that CsSCR is likely to act in tissue radial organization during somatic embryogenesis and root development.
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