1. Certain characteristics of human lymphocyte migration inhibitory factors (LyMIF) and the effect of ampicillin on their appearance in unstimulated and PHA-stimulated cultures.
- Author
-
Skopińska-Rózewska E, Madaliński W, and Lukasik R
- Subjects
- Ampicillin administration & dosage, Cells, Cultured, Dose-Response Relationship, Drug, Drug Synergism, Granulocytes metabolism, Hot Temperature, Humans, In Vitro Techniques, Lymphocytes metabolism, Molecular Weight, Phytohemagglutinins pharmacology, Ampicillin pharmacology, Chemotaxis, Leukocyte drug effects, Leukocyte Migration-Inhibitory Factors physiology, Lymphokines physiology
- Abstract
Supernatants of human blood mononuclear cells stimulated with PHA, contained factors inhibitory for in vitro migration of human lymphocytes and granulocytes. After ultrafiltration of supernatants through Amicon PM-10 some stimulatory activity appeared in the bottom fraction. Sephadex G-100 gel filtration chromatography of supernatants showed three zones of lymphocyte migration inhibitory activity: In the range of molecules of m.w. 35 000-90 000, heat-stable; Factors of m.w. from several to from ten to twenty thousand daltons, heat unstable; Low molecular weight substances, resistant to heat. The possible relationship of these factors to lymphotoxins, soluble lymphocyte T receptors for SRBC, lymphocyte chemotactic factor and prostaglandins is discussed. Ampicillin in doses of 10, 20 and 50 micrograms/ml potentiated both the development of lymphocyte migration inhibitory factors and the production of factors with an opposing effect (stimulating lymphocyte migration).
- Published
- 1983