1. Detection of selected arboviral infections in patients with history of persistent fever in Pakistan
- Author
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Muhammad Zubair Shabbir, Gabriel Defang, Nadia Mukhtar, Ehab Amir, Zarfishan Tahir, Tariq Abbas, and Tahir Yaqub
- Subjects
Serotype ,Adult ,Male ,Hemorrhagic Fevers, Viral ,Veterinary (miscellaneous) ,030231 tropical medicine ,Population ,Enzyme-Linked Immunosorbent Assay ,medicine.disease_cause ,Antibodies, Viral ,Real-Time Polymerase Chain Reaction ,Virus ,Dengue fever ,03 medical and health sciences ,Young Adult ,0302 clinical medicine ,Sex Factors ,Medicine ,Humans ,Pakistan ,030212 general & internal medicine ,Chikungunya ,Seroconversion ,education ,Antigens, Viral ,education.field_of_study ,Disease surveillance ,biology ,business.industry ,Middle Aged ,medicine.disease ,Virology ,Infectious Diseases ,Insect Science ,Population Surveillance ,biology.protein ,Parasitology ,Female ,Seasons ,Antibody ,business - Abstract
Surveillance is a valuable tool for understanding prevailing and previously undiagnosed infections in a geographic area. We examined 480 archived serum samples from patients with history of persistent fever (>40°C, 60-72h) who were referred to hospitals in Rawalpindi/Islamabad, Lahore, and Faisalabad districts for dengue antibody detection in 2014-15. Each sample was processed for detection of antigens and seroconversion, using real-time polymerase chain reaction and enzyme linked immunosorbent assay, respectively, against dengue haemorrhagic fever (DHF) virus serotypes 1-4, West Nile virus fever (WNVF), Crimean-Congo haemorrhagic fever (CCHF), and Chikungunya virus (CGV). The presence of antigens and antibodies to at least one of the studied viral haemorrhagic fevers (VHFs) was detected in 465 (96.8%, 95% CI: 94.9-98.1) and 442 samples (92.1%, 95% CI: 89.3-94.2), respectively. No sera were found positive to CCHF. There was a significant association between gender and positivity to at least one of the VHFs (χ2=8.12, df=1, p
- Published
- 2017