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The contribution concentrates on a special form of food sale. It solves several attributes of connection of food sale and electronic commerce. Firstly it describes experiences got by foreign companies like Tesco or Ahold – leaders of food commerce. Strategies and achievements in the field of electronic commerce of theirs were introduced.Following part of the work is devoted to leading Czech e-shops. Vltava e-shop and e-shops of its group (Cybex.cz and others) were assessed. Several market researches were introduced (about number of internet accesses in the Czech Republic, about time dedicated to PC and internet, knowing e-commerce or its use in practice). In the last part of the first section are mentioned foreign e-shop targeting Czech e-commerce market.In the third part are advantages of e-shopping summarized. Time saving, opening time, goods comparisons are briefly mentioned.The application part was aimed to Jednota’s future e-shop. That means that existing retail chain was chosen. So the foundation of e-shop is rather easier. Than the assortment of Czech e-shops was described, especially in connection with customers’ preferences. Customers’ shopping behavior was exa­mi­ned. So possible ways out could be founded.Following part takes care of implementation of food e-shop. Supply, logistic solution, payment solution, strategy of gaining and maintenance customers, contracting, information technologies, solutions and investment return were mentioned or solved.Results of the contribution are summarized in the conclusion. Relationship between theory of e-commerce and its application was highlighted as a necessary condition of its functioning in real world.