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The aim of this study was to compare insulin responsevness to acute glucose infusion in cows of Holstein Friesian (HF) and Buša breeds during the peripartal period. Eight cows per each group (HF and Buša), were chosen. At day 7 prior to calving (ante partum) and day 14 after calving (post partum) animals were subjected to a glucose tolerance test (GTT). Blood samples were taken immediately before infusion and 15, 30, 60, 120 and 180 min thereafter. Glucose and insulin concentrations were measured in each blood sample, while BHBA and NEFA were measured only in samples taken before the infusion. QUICKY an indicator of insulin resistance in cows was calculated. Basal glycemia did not significantly differ between the breeds. Basal insulinemia was significantly higher in Buša than in HF cows in both examined periods (p