1. PUFA-dependent alteration of oxidative parameters of a canine mastocytoma cell line
- Author
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Sandra Schmutzler, Herbert Fuhrmann, Julia Schumann, and L. Bachmann
- Subjects
Linoleic acid ,Wasp Venoms ,Canine Mastocytoma ,Biology ,Lipid peroxidation ,chemistry.chemical_compound ,Dogs ,Cell Line, Tumor ,medicine ,Animals ,Mast Cells ,chemistry.chemical_classification ,General Veterinary ,Mastocytoma ,medicine.disease ,Mast cell ,Eicosapentaenoic acid ,medicine.anatomical_structure ,Biochemistry ,chemistry ,Fatty Acids, Unsaturated ,Intercellular Signaling Peptides and Proteins ,Arachidonic acid ,Lipid Peroxidation ,Peptides ,Reactive Oxygen Species ,Oxidation-Reduction ,DNA Damage ,Polyunsaturated fatty acid - Abstract
Mast cells play a key role in the immune response. Thereby, the balance of oxidative metabolism is of importance in mast cell mediator synthesis and release. Fatty acids may modify mast cell function in several ways. In this study, we investigated the influence of polyunsaturated fatty acids (PUFAs) on oxidative parameters of a canine mastocytoma cell line. C2 cells were cultured in media supplemented with linoleic acid, arachidonic acid, alpha-linolenic acid and eicosapentaenoic acid, respectively. Production of reactive oxygen species (ROS) as well as lipid peroxides was tested. Furthermore, stressor-induced DNA damage was measured. Exposure of the cells to PUFAs resulted in a significant increase in the synthesis of both ROS and lipid peroxides. Distinct differences between the PUFAs tested underline the impact of the unsaturation degree of fatty acids as well as the position of double bonds on mast cells.
- Published
- 2010