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Powder Bed Fusion - Laser Beam (PBF-LB) parts experience a significant decline in fatigue performance when process-induced defects are present. In this work, a decline in 4-point bend fatigue life was observed in PBF-LB Ti-6Al-4V coupons fabricated at constant power with increasing scanning velocity and which underwent subsequent stress relief and surface machining. Specifically, the presence of pores that resemble lack-of-fusion (LoF) and a decline in fatigue life were observed at scanning velocities lower than that expected from prior published work. It was hypothesized that this unexpected presence of LoF pores resulted from melt pool geometry variability that was not considered in prior work when the LoF criterion was implemented. Further, these pores can be small in size and infrequent in their occurrence when the melt pool geometry variability is not severe. Such sparse pores are challenging to characterize using conventional 2D characterization methods. This work leverages tall and narrow coupon geometry and high-resolution X-ray micro computed tomography (X-μCT) to capture LoF porosity. The results show that a modified melt pool overlap-based LoF criterion considering melt track variability captures the unexpected occurrence of LoF pores observed in X-μCT. In addition, the LoF percent metric displays a strongly negative correlation with fatigue performance. The insights from this work provide guidance on characterizing melt pool geometry variability across scan lines to systematically evaluate processing parameters that generate LoF pores, which, in turn, could lower fatigue performance.