1. Long‐Term Observation of Locomotion of Drosophila Larvae Facilitates Feasibility of Food‐Choice Assays
- Author
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Jonas Bittern, Marie Baldenius, Christian Klämbt, and Marit Praetz
- Subjects
fungi ,Biomedical Engineering ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Term (time) ,Biomaterials ,Food Preferences ,Larva ,Food choice ,Biological neural network ,Animals ,Feasibility Studies ,Drosophila ,Animal behavior ,Adaptation ,human activities ,Neuroscience ,Locomotion ,Drosophila larvae - Abstract
Animal behavior is reflected by locomotor patterns. To decipher the underlying neural circuitry locomotion has to be monitored over often longer time periods. Here a simple adaptation is described to constrain movement of third instar Drosophila larvae to a defined area and use Frustrated total internal reflection based imaging method (FIM) imaging to monitor larval movements up to 1 h. It is demonstrated that the combination of FIM imaging and long analysis periods facilitates the conduction of food choice assays and provides the means to easily quantify food preferences.
- Published
- 2021
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