1. Effects of Zinc on Hepatopancreatic Cell Culture of Kuruma Prawn, Litopenaeus vannamei
- Author
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Da Li, Hai Ming Xu, Hui Wang, Chun Long Zhao, Hong Wei Wang, and Duan Bo Cai
- Subjects
biology ,Cell division ,Chemistry ,General Engineering ,Litopenaeus ,biology.organism_classification ,Molecular biology ,Penicillin ,Cell culture ,medicine ,Prawn ,Hepatopancreas ,Subculture (biology) ,Primary cell ,medicine.drug - Abstract
The hepatopancreatic cell culture of the kuruma prawn, Litopenaeusvannamei, was conducted to identify the effects of zinc on cell division. The culturesystem consists of medium 199 (M 199) supplemented with 0.060 mol/L NaCl,1.011g/L glucose, 1000 UI/ml penicillin, 1000 μg/ml treptomycin, 20% heatinactivated fetal calf serum (FCS) for primary cells and 10 % for subculture cells. TheRNA/DNA ratio in cultured cells was measured. The results show that the celldivision of cultured hepatopancreas cells in L. vannamei was increased by the optimalconcentration of Zn2+, 80 μg/L.
- Published
- 2013