1. Effect on JNK/MAPK Pathways in the Damage of Rat Testicle Sertoli Cells Induced by DBP
- Author
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Peng Min Wang, Huan Li, Yi Zhang, and Jing Hong Yu
- Subjects
MAPK/ERK pathway ,Messenger RNA ,medicine.diagnostic_test ,biology ,Chemistry ,Dibutyl phthalate ,General Engineering ,Vimentin ,Sertoli cell ,Molecular biology ,chemistry.chemical_compound ,medicine.anatomical_structure ,Western blot ,Endocrine disrupting compound ,Apoptosis ,medicine ,biology.protein - Abstract
Dibutyl phthalate (DBP), an accepted environmental endocrine disrupting compound, which has anti-androgenic effects,is widely used in many industries such as plastics, pharmaceuticals, plastic food packaging materials. This article intends to study the effect on JNK/MAPK pathways in the damage of rat testicle sertoli cells induced by DBP via vitro test. Testicular sertoli cells of rates were incubated by medium containing DBP in different concentrations (0μg/ml,1μg/ml,10μg/ml, 100μg/ml). after culturing for 24 hours the expression of vimentin was detected by immunofluorescence; the expression of vimentin mRNA detected by RT-PCR; Western blot analyzed the expression of related protein of JNK/MAPK pathways in sertoli cells. The results showed that vimentin highly express in sertoli cells and gradually decrease with the increase of poisonous doses. The mRNA expression of vimentin in each exposure group has a downtrend with the increase of does. The total protein of JNK and P-JNK protein increased gradually in a dose effect manner. These results suggested that DBP can decrease the expression of vimentin mRNA, the decrease of which was regulated by JNK/MAPK signal transduction pathway that activated by DBP, and then induced the apoptosis of Sertoli cell.
- Published
- 2013