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The purpose of this study is to investigate the effect of fucosterol on the induction of apoptosis and the molecular mechanism involved in Human promyelocytic leukemia HL-60 Cells. HL-60 Cells were treated with different concentrations of fucosterol at different time. MTT method was used to study fucosterol anti-tumor activity. Morphology observation was performed to determine the effects of fucosterol on apoptosis of HL-60 cells. Flow cytometry (FCM) was used to detect the cell cycle. Laser scanning confocal microscope (LSCM) was used to analyze the expressions of Fas, FasL, Fadd and Caspase-8. Caspase activity kits were used to determine the activity of Caspase-8 and Caspase-3. The results showed fucosterol could inhibit the growth of HL-60 cells, and the apoptosis morphology for 48 h treatment was obvious, which showed cell protuberance, cytoplasm concentrated and apoptotic body. Fucosterol treatment for 24 h increased the protein expression of Fas, FasL, Fadd and Caspase-8. It also showed that the activity of Caspase-3 and Caspase-8 has increased significantly. In conclusion, Fucosterol could induce HL-60 cells apoptosis via death receptor pathway.

The purpose of this study is to investigate the effect of fucosterol on the induction of apoptosis and the molecular mechanism involved in Human promyelocytic leukemia HL-60 Cells. HL-60 Cells were treated with different concentrations of fucosterol at different time. MTT method was used to study fucosterol anti-tumor activity. Morphology observation was performed to determine the effects of fucosterol on apoptosis of HL-60 cells. Flow cytometry (FCM) was used to detect the cell cycle. Laser scanning confocal microscope (LSCM) was used to analyze mitochondrial membrane potential (MMP). Western blot was performed to analyze the expressions of Cyt-C, Caspase-9 and Caspase-3. The results showed fucosterol could inhibit the growth of HL-60 cells, and the apoptosis morphology for 48 h treatment was obvious, which showed cell protuberance, cytoplasm concentrated and apoptotic body. Fucosterol treatment for 24 h decreased MMP in dose-dependent manners. It also induced the release of Cyt-C and the activation of Caspase-9 and-3. In conclusion, Fucosterol could induce HL-60 cells apoptosis through a mitochondrial pathway.

To study the effect of chelidonine on Cdk1, p-Cdk1( Thr14), cyclinB1 protein expression in SGC-7901 Cells, and to explore the mechanism of chelidonine inducing G2/M phase arrest in SGC-7901 Cells. Western Blotting was used to study the effects of chelidonine on the Cdk1, p-Cdk1(Thr14) and cyclinB1 protein expressions in SGC-7901 cells. Chelidonine could significantly decrease the expression of Cdk1 and cyclinB1 protein. Meanwhile, chelidonine could remarkably increase the expression of p-Cdk1(Thr14) protein in a dose dependent manner. Chelidonine can down-regulate the expression of Cdk1, cyclinB1 and up-regulate p-Cdk1 (Thr14) protein expression in SGC-7901 cells,which may be one of the role mechanism of G2 /M phase arrest induced by chelidonine in SGC-7901 cells which may be one of the role mechanism of G2 /M phase arrest induced by chelidonine in SGC-7901 Cells.

To study the mitotic catastrophe induction effect of chelidonine on human gastric carcinoma SGC-7901 cells, MTT assay was applied to detect the proliferation inhibition effect of chelidonine. Giemsa staining was used to observe the morphological changes of the nucleus in gastric cancer cells and mitotic index (MI) was calculated. The results showed that chelidonine could effectively inhibit the proliferation of SGC-7901 cells and the IC50 was 48.67μmol/L. After treatment with 6.25-25μmol/L chelidonine, the numbers of giant cells with two or more nuclei was increased along with the increase of drug concentration. The mitotic index of cells was significantly increased compared with that of the control group (P

To study Asparagus officinalis polysaccharide on the function of erythrocyte in S180 mice on immunological reaction of lymphocyte. Suspensions of lymphocytes and autologous plasma were separated from whole blood of healthy mice. The erythrocytes were separated from whole blood of Asparagus officinalis polysaccharide mice. Using the autologous plasm as reactive medium, the role of erythrocytes in regulating the immunological reaction of lymphocytes was mesured. The expression of CD25 on lymphocytes was measured by flow cytometry. The immunological regulating ability of erythrocyte in control group is much lower than normal group, and in Asparagus officinalis polysaccharide group is much higher than control group. According to the effects of erythrocyte CD35 on the immune-response of lymphocyte and the different of the expression of CD25 on lymphocytes, we prove that Asparagus officinalis polysaccharide can improve the erythrocyte function of S180 mice.

To explore the influence of Asparagus polysaccharide on Band 3 proteins and glycophorin A(GPA) of S180 mice erythrocyte membrane by contrasting the groups treated with deferent ways,The experiment was equipped with 5 groups: normal control group (physiological saline), negative control group (physiological saline), Asparagus polysaccharide group (25mg/kg), Asparagus polysaccharide group (50mg/kg) and Asparagus polysaccharide group(100mg/kg).The content of sialic acid(SA) was determined by spectrophotometric method; the negative charges were detected by high performance capillary electrophoresis(HPCE); the relative content of glycophorin A and Band 3 were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE; anion transportation function of Band 3 was examined by the fluorescence spectrophotometric method;Flow Cytometer(FCM) was needed to observe the potential of erythrocyte membrane. In the result, Asparagus polysaccharide increased the content of sialic acid; advanced the migration time; improved the relative content of GPA and Band 3; obviously enhanced the CI- transportation function of erythrocyte Band 3; and caused the membrane potential obviously to elevate, Asparagus polysaccharide has the simultaneously influence on the functions of glycophorin A and Band 3 which are closely connected. It is assumed that the anti-tumor function of Asparagus polysaccharide partly concerns function adjustments of glycophorin A and Band 3 from S180 mice erythrocyte membrane.

Solanine is one of chemicalcomponents in the tuber and the sprout of the potato which is toxic to human. Some studies on the toxicity of solanine on humans and animals have been reported, little is known about the mechanism of its testicular toxicity. In present study, the toxicity of solanine on male reproductive function was investigated in adult male Kunming mice. Compared with the control group, there was an obvious pathological change in testis, and the expression levels of 3β-HSD and vimentin decreased when the test concentration of solanine was at 21 mg/kg/day. Meanwhile, there was a significant dose- and duration-dependent reduction in the testicular weight and organ coefficient. However, no changes have been detected about the level of testosterone and there was a dramatic increase in the expression of LH in Leydig cells. Results of this study suggested that solanine leaded to male reproductive toxicity influencing the functions of Sertoli cells and Leydig cells.

To investigate the mechanism on Capparis spionosa L polysaccharide(CSPS) inducing apoptosis in HepG2 human hepatoma cell. MTT was ddopted to determine if CSPS had cytotoxic effect on HepG2. Morphology of HepG2 changed with dosages of CSPS was detected by laser confocal scanning microscope. Flow cytometry(FCM) was used to detect the apoptosis by PI labeling method. Calcium, mitochondrial membrane potential, Bcl-2/Bax of HepG2 cells were detected by laser confocal scanning microscope. The result of MTT showed that CSPS could inhibit the growth of HepG2 significantly. HepG2 cells were shrinkage, fragmentation, appearance of apoptotic bodies by laser confocal scanning microscope. HepG2 cell apoptosis rate was increased gradually with dosage by FCM. Calcium concentration, mitochondrial membrane potential, Bcl-2 protein of HepG2 were decreased, Bax protein content of HepG2 was increased by laser confocal scanning microscope. CSPS induced HepG2 apoptosis by controlling Bax/Bcl-2 in Ca2+ path.

To investigate the absorption of ginsenosides Rg1and Rb1 in Bazhen Decoction at different intestine segments of rats and the influence of the drug solution concentration, pH, P-gp inhibitor on ones. The intestine cannulation was performed for in situ recirculation. The concentration of ginsenosides Rg1 and Rb1, and phenol red in the flux were measured by the reversed phase HPLC and ultraviolet spectrophotometry, respectively. The absorption of ginsenosides Rg1 and Rb1 in Bazhen Decoction in intestine of rat show first-order kinetics with the absorption mechanism of passive diffusion. Ginsenoside Rg1 has specific absorption locus of duodenum and ginsenoside Rb1 has specific absorption locus of jejunum. Meanwhile, ginsenoside Rb1 is the P-gp substrate, and could increase its fraction of bioavailability by corporation with P-gp inhibitor.

In order to investigate the effect and its mechanism of esculetin on human gastric carcinoma SGC-7901 cells, the effect of esculetin on human gastric carcinoma SGC-7901 cells were investigated by MTT method in vitro studies. Then the morphological feature changes of apoptosis cells with esculetin of different concentration by transmission electron microscope were observed and the levels of Cty-c in SGC-7901 cells line using FCM method were analyzed. The results revealed that the Esculetin significantly inhibited the growth of human gastric carcinoma SGC-7901 cells and greatly decreased its growth rate with the esculetin concentration increased, and then the morphological feature changes of apoptosis cells had been observed. After 24h, esculetin could make that the concentration of Cty-c in endochylema and Caspase-3 were all increased. Thus the study showed that esculetin remarkably inhibited the growth of human gastric carcinoma SGC-7901 cells and induce its apoptosis.

To optimize the sulfated modification conditions of Undaria pinnatifida polysaccharides (UPPSⅠB). UPPSⅠB was modified by chlorosulfonic acid-pyridine method. L9(34) orthogonal test was used to optimize the reaction temperature, reagent ratio and reaction time with the degree of sulfate group and carbohydrate content as index. The major factor was reaction temperature. The optimal modification conditions were that the reaction temperature was 60°C, the ratio of chlorosulfonic acid to pyridine was 1:2, and the reaction time was 1.5h. The sulfated UPPSⅠB could be obtained using the optimal modification conditions.

To investigate extracting technique and potential biological activity of fucoidin in Undaria pinnatifid, a kind of marine organisms. Factors such as solid-liquid ratio (times), time of extraction (min) and temperature of extraction (°C) were chosen to be investigated to examine the best extracting technique. 50 mice were randomed to negative comparison group, positive comparison group and experiment groups. Mice were inoculated tumor stock S180. The negative comparison group were administrated with physiological saline, positive comparison group administrated with astragalus polyose(50mg/(kg•d)), and experiment groups administrated with fucoidin(100mg/(kg•d), 50mg/(kg•d), 25mg/(kg•d)). The tumors were peeled and weighted after 7 days. The best extracting condition is definite to be 100 °C, 60 times of water and 7h. The content of fucoidin in Undaria pinnatifida is 55.4％ after preliminary purification. The tumor inhibition rate of experiment groups are 56.19%, 65.71% and 30.48%. The results of investigation on orthogonal experiments are reliable. And fucoidin in Undaria pinnatifida has certain antitumor effect.

Study on whether genetic toxicity of toluene diisocyanate (TDI) is reversible. This paper detected Chromosomal aberrations and content of RNA & DNA. Chromosome aberration rate and the RNA/DNA ratio of TDI 1/4LC50 and 1/2 LC50 dosing exposure group were higher than negative control group significantly (P0.05).The results showed that the damage of TDI on chromosomes and DNA was repairable, but can not be repaired completely.

To investigate the combined effect of kappa-selenocarrageenan (KSC) and paclitaxol (Taxol) on MCF-7 cells in vitro. The cytotoxic effect of both drugs on MCF-7 cells was measured by MTT assay, and the King’s principle was used to evaluate the interaction of drugs combination. KSC and Taxol alone have significant growth inhibitory effects on HepG-2 cells in dose- and time-dependent manners, and the combination groups show more remarkable anti-proliferative activities than the two drugs alone. The IC50 values of KSC and Taxol alone on HepG-2 cells for 72h are 48.445 mg/L and 2.592 nmol/L, respectively. While combining with 30 mg/L KSC, IC50 value of Taxol decreases sharply to 0.353 nmol/L. Q values of their combination are 0.908~1.051, which demonstrates additive effect. In short, selenium polysaccharide has striking inhibition effect on MCF-7 cells and can enhance efficacy of chemotherapeutic agents and reduce their side effects.