9 results
Search Results
2. Human MHC Class II and Invariant Chain Knock‐in Mice Mimic Rheumatoid Arthritis with Allele Restriction in Immune Response and Arthritis Association.
- Author
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Romero‐Castillo, Laura, Li, Taotao, Do, Nhu‐Nguyen, Sareila, Outi, Xu, Bingze, Hennings, Viktoria, Xu, Zhongwei, Svensson, Carolin, Oliveira‐Coelho, Ana, Sener, Zeynep, Urbonaviciute, Vilma, Ekwall, Olov, Burkhardt, Harald, and Holmdahl, Rikard
- Subjects
RHEUMATOID arthritis ,IMMUNE response ,T cells ,B cells ,TRANSGENIC mice ,ALLELES ,ARTHRITIS ,B cell receptors - Abstract
Transgenic mice expressing human major histocompatibility complex class II (MHCII) risk alleles are widely used in autoimmune disease research, but limitations arise due to non‐physiologic expression. To address this, physiologically relevant mouse models are established via knock‐in technology to explore the role of MHCII in diseases like rheumatoid arthritis. The gene sequences encoding the ectodomains are replaced with the human DRB1*04:01 and 04:02 alleles, DRA, and CD74 (invariant chain) in C57BL/6N mice. The collagen type II (Col2a1) gene is modified to mimic human COL2. Importantly, DRB1*04:01 knock‐in mice display physiologic expression of human MHCII also on thymic epithelial cells, in contrast to DRB1*04:01 transgenic mice. Humanization of the invariant chain enhances MHCII expression on thymic epithelial cells, increases mature B cell numbers in spleen, and improves antigen presentation. To validate its functionality, the collagen‐induced arthritis (CIA) model is used, where DRB1*04:01 expression led to a higher susceptibility to arthritis, as compared with mice expressing DRB1*04:02. In addition, the humanized T cell epitope on COL2 allows autoreactive T cell‐mediated arthritis development. In conclusion, the humanized knock‐in mouse faithfully expresses MHCII, confirming the DRB1*04:01 alleles role in rheumatoid arthritis and being also useful for studying MHCII‐associated diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
3. Nanoparticles Synergize Ferroptosis and Cuproptosis to Potentiate Cancer Immunotherapy.
- Author
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Li, Youyou, Liu, Jing, Chen, Yimei, Weichselbaum, Ralph R., and Lin, Wenbin
- Subjects
PROGRAMMED death-ligand 1 ,TRIPLE-negative breast cancer ,IMMUNOTHERAPY ,NANOPARTICLES ,IMMUNE checkpoint proteins ,T cells - Abstract
The recent discovery of copper‐mediated and mitochondrion‐dependent cuproptosis has aroused strong interest in harnessing this novel mechanism of cell death for cancer therapy. Here the design of a core‐shell nanoparticle, CuP/Er, for the co‐delivery of copper (Cu) and erastin (Er) to cancer cells for synergistic cuproptosis and ferroptosis is reported. The anti‐Warburg effect of Er sensitizes tumor cells to Cu‐mediated cuproptosis, leading to irreparable mitochondrial damage by depleting glutathione and enhancing lipid peroxidation. CuP/Er induces strong immunogenic cell death, enhances antigen presentation, and upregulates programmed death‐ligand 1 expression. Consequently, CuP/Er promotes proliferation and infiltration of T cells, and when combined with immune checkpoint blockade, effectively reinvigorates T cells to mediate the regression of murine colon adenocarcinoma and triple‐negative breast cancer and prevent tumor metastasis. This study suggests a unique opportunity to synergize cuproptosis and ferroptosis with combination therapy nanoparticles to elicit strong antitumor effects and potentiate current cancer immunotherapies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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4. Tumor Exosomal ENPP1 Hydrolyzes cGAMP to Inhibit cGAS‐STING Signaling.
- Author
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An, Yu, Zhu, Jinchao, Xie, Qihui, Feng, Jianzhou, Gong, Yanli, Fan, Qian, Cao, Jiao, Huang, Zhi, Shi, Weixiong, Lin, Qingyuan, Wu, Lingling, Yang, Chaoyong, and Ji, Tianhai
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EXOSOMES ,T cells ,TUMORS ,TUMOR microenvironment ,LUNG cancer ,VENOM - Abstract
To evade immune surveillance, tumor cells express ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1) on the surface of their membrane, which degrades extracellular cyclic GMP‐AMP (cGAMP), thereby inhibiting the cyclic GMP‐AMP synthase (cGAS) stimulator of interferon gene (STING) DNA‐sensing pathway. To fully understand this tumor stealth mechanism, it is essential to determine whether other forms of ENPP1 with hydrolytic cGAMP activity also are present in the tumor microenvironment to regulate this innate immune pathway. Herein, it is reported that various tumor‐derived exosomes carry ENPP1, and can hydrolyze synthetic 2′3′‐cGAMP and endogenous 2′3′‐cGAMP produced by cells to inhibit cGAS‐STING pathway in immune cells. Moreover, tumor exosomal ENPP1 also can hydrolyze 2′3′‐cGAMP bound to LL‐37 (an effective transporter of 2′3′‐cGAMP) to inhibit STING signaling. Furthermore, high expression of ENPP1 in exosomes is observed isolated from human breast and lung cancer tissue, and tumor exosomal ENPP1 inhibited the immune infiltration of CD8+ T cells and CD4+ T cells. The results elucidate the essential function of tumor exosomal ENPP1 in the cGAS‐STING pathway, furthering understanding of the crosstalk between the tumor cells and immune system. [ABSTRACT FROM AUTHOR]
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- 2024
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- View/download PDF
5. Urolithin A Hijacks ERK1/2‐ULK1 Cascade to Improve CD8+ T Cell Fitness for Antitumor Immunity.
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Ma, Shuaiya, Wu, Qi, Wu, Wenxian, Tian, Ye, Zhang, Jie, Chen, Chaojia, Sheng, Xue, Zhao, Fangcheng, Ding, Lu, Wang, Taixia, Zhao, Laixi, Xie, Yuying, Wang, Yongxiang, Yue, Xuetian, Wu, Zhuanchang, Wei, Jian, Zhang, Kun, Liang, Xiaohong, Gao, Lifen, and Wang, Hongyan
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T cells ,CYTOTOXIC T cells ,CHIMERIC antigen receptors ,REACTIVE oxygen species ,IMMUNITY ,CD8 antigen ,OXYGEN consumption - Abstract
According to the latest evidence, the microbial metabolite Urolithin A (UA), known for its role in promoting cellular health, modulates CD8+ T cell‐mediated antitumor activity. However, the direct target protein of UA and its underlying mechanism remains unclear. Here, this research identifies ERK1/2 as the specific target crucial for UA‐mediated CD8+ T cell activation. Even at low doses, UA markedly enhances the persistence and effector functions of primary CD8+ cytotoxic T lymphocytes (CTLs) and human chimeric antigen receptor (CAR) T cells both in vitro and in vivo. Mechanistically, UA interacts directly with ERK1/2 kinases, enhancing their activation and subsequently facilitating T cell activation by engaging ULK1. The UA‐ERK1/2‐ULK1 axis promotes autophagic flux in CD8+ CTLs, enhancing cellular metabolism and maintaining reactive oxygen species (ROS) levels, as evidenced by increased oxygen consumption and extracellular acidification rates. UA‐treated CD8+ CTLs also display elevated ATP levels and enhanced spare respiratory capacity. Overall, UA activates ERK1/2, inducing autophagy and metabolic adaptation, showcasing its potential in tumor immunotherapy and interventions for diseases involving ERKs. [ABSTRACT FROM AUTHOR]
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- 2024
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6. Tumor‐Associated Monocytes Reprogram CD8+ T Cells into Central Memory‐Like Cells with Potent Antitumor Effects.
- Author
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Yang, Zeliang, Liu, Liang, Zhu, Zhenyu, Hu, Zixi, Liu, Bowen, Gong, Jingjing, Jin, Yuan, Luo, Juan, Deng, Yichen, Jin, Yan, Wang, Guangxi, and Yin, Yuxin
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T cells ,T-cell exhaustion ,MONOCYTES ,NITRIC-oxide synthases ,IMMUNOLOGIC memory ,MEMBRANE proteins - Abstract
CD8+ T cells are critical for host antitumor responses, whereas persistent antigenic stimulation and excessive inflammatory signals lead to T cell dysfunction or exhaustion. Increasing early memory T cells can improve T cell persistence and empower T cell‐mediated tumor eradication, especially for adoptive cancer immunotherapy. Here, it is reported that tumor‐associated monocytes (TAMos) are highly correlated with the accumulation of CD8+ memory T cells in human cancers. Further analysis identifies that TAMos selectively reprogram CD8+ T cells into T central memory‐like (TCM‐like) cells with enhanced recall responses. L‐NMMA, a pan nitric oxide synthase inhibitor, can mitigate TAMo‐mediated inhibition of T cell proliferation without affecting TCM‐like cell generation. Moreover, the modified T cells by TAMo exposure and L‐NMMA treatment exhibit long‐term persistence and elicit superior antitumor effects in vivo. Mechanistically, the transmembrane protein CD300LG is involved in TAMo‐mediated TCM‐like cell polarization in a cell‐cell contact‐dependent manner. Thus, the terminally differentiated TAMo subset (CD300LGhighACElow) mainly contributes to TCM‐like cell development. Taken together, these findings establish the significance of TAMos in boosting T‐cell antitumor immunity. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
7. Melanoma Derived Exosomes Amplify Radiotherapy Induced Abscopal Effect via IRF7/I‐IFN Axis in Macrophages.
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Wang, Lu, Shen, Kangjie, Gao, Zixu, Ren, Ming, Wei, Chenlu, Yang, Yang, Li, Yinlam, Zhu, Yu, Zhang, Simin, Ding, Yiteng, Zhang, Tianyi, Li, Jianrui, Zhu, Ming, Zheng, Shaoluan, Yang, Yanwen, Du, Shisuo, Wei, Chuanyuan, and Gu, Jianying
- Subjects
EXOSOMES ,FLUORESCENCE in situ hybridization ,TYPE I interferons ,MACROPHAGES ,T cells ,DOSE-response relationship (Radiation) - Abstract
Radiotherapy (RT) can induce tumor regression outside the irradiation field, known as the abscopal effect. However, the detailed underlying mechanisms remain largely unknown. A tumor‐bearing mouse model is successfully constructed by inducing both subcutaneous tumors and lung metastases. Single‐cell RNA sequencing, immunofluorescence, and flow cytometry are performed to explore the regulation of tumor microenvironment (TME) by RT. A series of in vitro assays, including luciferase reporter, RNA Pulldown, and fluorescent in situ hybridization (FISH) assays, are performed to evaluate the detailed mechanism of the abscopal effect. In addition, in vivo assays are performed to investigate combination therapy strategies for enhancing the abscopal effect. The results showed that RT significantly inhibited localized tumor and lung metastasis progression and improved the TME. Mechanistically, RT promoted the release of tumor‐derived exosomes carrying circPIK3R3, which is taken up by macrophages. circPIK3R3 promoted Type I interferon (I‐IFN) secretion and M1 polarization via the miR‐872‐3p/IRF7 axis. Secreted I‐IFN activated the JAK/STAT signaling pathway in CD8+ T cells, and promoted IFN‐γ and GZMB secretion. Together, the study shows that tumor‐derived exosomes promote I‐IFN secretion via the circPIK3R3/miR‐872‐3p/IRF7 axis in macrophages and enhance the anti‐tumor immune response of CD8+ T cells. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
8. Protein Kinase STK24 Promotes Tumor Immune Evasion via the AKT‐PD‐L1 Axis.
- Author
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Wang, Ning, Jiang, Yu, Li, Mengjie, Wang, Haofei, Pan, Jie, Tang, Yang, Xie, Shaofang, Xu, Yunyang, Li, Xu, Zhou, Xuefei, Xu, Pinglong, Lin, Wenlong, and Wang, Xiaojian
- Subjects
PROTEIN kinases ,CYTOTOXIC T cells ,KILLER cells ,TUMOR growth ,T cells - Abstract
Immunotherapy targeting PD‐L1 is still ineffective for a wide variety of tumors with high unpredictability. Deploying combined immunotherapy with alternative targeting is practical to overcome this therapeutic resistance. Here, the deficiency of serine‐threonine kinase STK24 is observed in tumor cells causing substantial attenuation of tumor growth in murine syngeneic models, a process relying on cytotoxic CD8+ T and NK cells. Mechanistically, STK24 in tumor cells associates with and directly phosphorylates AKT at Thr21, which promotes AKT activation and subsequent PD‐L1 induction. Deletion or inhibition of STK24, by contrast, blocks IFN‐γ‐mediated PD‐L1 expression. Various murine models indicate that in vivo silencing of STK24 can significantly enhance the efficacy of the anti‐PD‐1 blockade strategy. Elevated STK24 levels are observed in patient specimens in multiple tumor types and inversely correlated with intratumoral infiltration of cytotoxic CD8+ T cells and with patient survival. The study collectively identifies STK24 as a critical modulator of antitumor immunity, which engages in AKT and PD‐L1/PD‐1 signaling and is a promising target for combined immunotherapy. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
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9. Pirin Inhibits FAS‐Mediated Apoptosis to Support Colorectal Cancer Survival.
- Author
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Ma, Huanhuan, Suleman, Muhammad, Zhang, Fengqiong, Cao, Tingyan, Wen, Shixiong, Sun, Dachao, Chen, Lili, Jiang, Bin, Wang, Yue, Lin, Furong, Wang, Jinyang, Li, Boan, and Li, Qinxi
- Subjects
COLORECTAL cancer ,COLON cancer ,COLON tumors ,T cells ,APOPTOSIS ,CD8 antigen ,CELL death - Abstract
Resistance to immunotherapy in colorectal cancer (CRC) is associated with obstruction of FAS (Apo‐1 or CD95)‐dependent apoptosis, a hallmark of cancer. Here it is demonstrated that the upregulation of pirin (PIR) protein in colon cancers promotes tumorigenesis. Knockout or inhibition of PIR dramatically increases FAS expression, FAS‐dependent apoptosis and attenuates colorectal tumor formation in mice. Specifically, NFκB2 is a direct transcriptional activator of FAS and robustly suppressed by PIR in dual mechanisms. One is the disruption of NFκB2 complex (p52‐RELB) association with FAS promoter, the other is the inhibition of NIK‐mediated NFκB2 activation and nuclear translocation, leading to the inability of active NFκB2 complex toward the transcription of FAS. Furthermore, PIR interacts with FAS and recruits it in cytosol, preventing its membrane translocation and assembling. Importantly, knockdown or knockout of PIR dramatically sensitizes cells to FAS mAb‐ or active CD8+ T cells‐triggered cell death. Taken together, a PIR‐NIK‐NFκB2‐FAS survival pathway is established, which plays a key role in supporting CRC survival. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
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