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Owing to the light weight and high fundamental frequency, timber floors exhibit impulse-like responses under human-induced excitation, which is different with the resonance-like responses for heavy concrete structures. The vibration serviceability of timber floors thus needs to be considered in a different manner. Many design codes for timber structures have required that the static displacement or dynamic response under human excitation should be limited within a threshold for the purpose of serviceability, while failing to provide appropriate method for predicting structural responses considering various affecting factors. Inspired by the idea of response spectrum, this paper proposed a design-oriented method for the peak acceleration prediction of high-frequency floors under human bouncing excitation. The prediction can be obtained for any desired confidence level. Statistical analysis shows that the acceleration responses are mostly dependent on structural fundamental frequency, structural damping ratio, and excitation frequency, which are considered in the proposed mathematical model. The application procedure and the experimental assessment of the proposed model are provided, showing the decent applicability of the proposed method. [ABSTRACT FROM AUTHOR]