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Hot pepper is the dominant vegetable crop grown in different parts of Ethiopia with long history of cultivation and considerable genetic diversity for most important morphological traits. However, shortage of varieties, the prevalence of fungal and bacterial as well as viral diseases, information is lacking on genetic diversity and genetic information to design genetic resource conservation to improve yield and yield components of hot pepper. The study was undertaken to assess the morphological diversity of 64 hot pepper genotypes at Axum Agricultural Research Centre in Mereb Leke District during the year 2017/2018, using 8×8 simple lattice design. Analysis of variance revealed that there were a significant (P