1. Evaluation of the HIV-1 reverse transcriptase inhibitory properties of extracts from some medicinal plants in Kenya
- Author
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James N Kanyara, Festus M. Tolo, Seiji Kageyama, C.N. Muthaura, Masahiko Kurokawa, G.M. Mungai, G.M. Rukunga, Rukia M Kibaya, John M Muli, Mawuli W. Kofi-Tsekpo, Peter M. Tukei, and K Shiraki
- Subjects
Foscarnet ,chemistry.chemical_classification ,Plants, Medicinal ,Traditional medicine ,Plant Extracts ,business.industry ,Human immunodeficiency virus (HIV) ,Positive control ,General Medicine ,medicine.disease_cause ,Kenya ,Reverse transcriptase ,chemistry.chemical_compound ,Enzyme ,chemistry ,Primer dimer ,HIV-1 ,Humans ,Reverse Transcriptase Inhibitors ,Medicine ,business ,Medicinal plants ,Thymidine triphosphate ,medicine.drug - Abstract
Extracts from twenty two medicinal plants popularly used in preparing traditional remedies in Kenya were screened for activity against the HIV-1 reverse transcriptase. The screening procedure involved the use of tritium labeled thymidine triphosphate as the enzyme substrate and polyadenylic acid.oligodeoxythymidylic acid [poly(rA).p(dT)12-18] as the template primer dimer. Foscarnet was used as a positive control in these experiments. At a concentration of 100µg/ml, extracts from eight of these plants showed at least 50 per cent reverse transcriptase inhibition. This activity was abitrarily considered as significant. This indicates that there is the probability that some antiretroviral compounds could be identified and isolated from materials from these plants. [Afr. J. Health Sci. 2002; 9:81-90]
- Published
- 2004
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