1. Geno protective and anti-apoptotic effect of green tea against perinatal lipopolysaccharide-exposure induced liver toxicity in rat newborns
- Author
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Jamaan S. Ajarem, Sami A. Gabr, Ahmad H. Alghadir, Revathi Sekar, Ahmed A. Allam, and Billy K. C. Chow
- Subjects
Lipopolysaccharides ,Liver Cirrhosis ,0301 basic medicine ,newborns ,Antioxidant ,Lipopolysaccharide ,Bilirubin ,green tea ,medicine.medical_treatment ,Apoptosis ,Green tea extract ,Pharmacology ,medicine.disease_cause ,Antioxidants ,Camellia sinensis ,Article ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,Pregnancy ,parasitic diseases ,Drug Discovery ,medicine ,Animals ,Rats, Wistar ,Tea ,Plant Extracts ,liver dysfunction ,lipopolysaccharide ,Albumin ,Polyphenols ,DNA ,Endotoxemia ,Oxidative Stress ,Perinatal Care ,030104 developmental biology ,Animals, Newborn ,Liver ,Complementary and alternative medicine ,chemistry ,green tea, lipopolysaccharide, liver dysfunction, apoptosis, newborns ,Maternal Exposure ,Immunology ,Female ,Chemical and Drug Induced Liver Injury ,Oxidative stress ,Phytotherapy - Abstract
Background: This study aims to examine the protective effect of green tea on the disturbances in oxidative stress and apoptosis related factors, mostly produced due to perinatal lipopolysaccharide (LPS) exposure, that subsequently induces liver cell damage. Materials and Methods: Anti-free radical, Antioxidant, scavenging, geno-protective, and antiapoptotic activity of aqueous green tea extract (AGTE) were assessed against LPS-induced hepatic dysfunction in newborn-rats. AGTE at doses of 100 & 200 mg/kg was orally administered daily to rat dams, during gestation and lactation. Results: AGTE was observed to exhibit protective effects by significantly attenuating LPS-induced alterations in serum AST, ALT, bilirubin, and albumin levels. Significant increase in the total antioxidant capacity (TAC), DNA contents, and reduction in nitric oxide (NO) levels were observed in AGTE treated rats comparing LPS-toxicated ones. Additionally, AGTE treatment significantly down-regulated apoptotic markers and this effect was directly correlated to the degree of hepatic fibrosis. The possible mechanisms of the potential therapeutic-liver protective effect of AGTE could be due to free radical scavenging potential and antiapoptotic properties caused by the presence of antioxidant polyphenolic components in AGTE. Conclusion: We thereby propose, based on our findings, that the anti-free radical and anti-apoptotic inducing properties of AGTE active constituents attribute to its functional efficacy as anti-fibrotic agent.
- Published
- 2017