1. Methylation status of cKi- ras and MHC genes in rat pituitary glands during aging and tumorigenesis
- Author
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J. Vijg, A. G. Uitterlinden, and P. E. Slagboom
- Subjects
Adenoma ,Aging ,Pituitary gland ,medicine.medical_specialty ,Genes, MHC Class I ,Biology ,Major histocompatibility complex ,medicine.disease_cause ,Methylation ,Internal medicine ,Isoschizomer ,MHC class I ,medicine ,Animals ,Pituitary Neoplasms ,Southern blot ,Rats, Inbred Strains ,DNA ,DNA, Neoplasm ,Molecular biology ,Rats ,Genes, ras ,medicine.anatomical_structure ,Endocrinology ,Pituitary Gland ,DNA methylation ,biology.protein ,Female ,Geriatrics and Gerontology ,Carcinogenesis - Abstract
Methylation patterns of MHC (major histocompatibility complex) class I and vKi-ras (viral Kirsten-ras) homologous sequences in normal and adenomatous rat pituitary glands were studied as a function of age by Southern hybridization analysis using the isoschizomers Hpa II and Msp I. Both MHC class I and vKi-ras homologous sequences were found to be hypomethylated in a number of tumors, compared to normal pituitary gland tissue. However, despite reports indicating a general demethylation in mammalian tissues in relation to donor age, age-related methylation changes in this apparently methylation-unstable and cancer-prone organ were not observed. (Aging 3: 141–146, 1991)
- Published
- 1991
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