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[Objective] The effect of bacteriostat (Qianxing No.1) on open tissue culture of sugarcane was discussed in this paper. [Method] The axillary bud explants of Qiantang No.5 were used as experimental material. Different concentrations of Qianxing No.1 were added in the media to determine the optimal bacteriostatic concentrations of Qianxing No.1 for induction and proliferation culture of sugarcane in the open tissue culture. [Result] The results showed Qianxing No.1 with the concentration of 0.5% could effectively inhibit the medium contamination. Under that bacteriostatic concentration, the surviving rate of explants was up to 80%, and the propagation coefficient was 3.1. [Conclusion] This research would provide a theoretic basis for the simplification of sugarcane tissue culture procedure and the reduction of production costs. [ABSTRACT FROM AUTHOR]