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Rice blast disease is one of the most devastating diseases in rice production, which severely affects the high and stable yield of rice. The formation of appressorium plays a key role in the pathogenesis of Magnaporthe grisea in rice. It has been confirmed that a P-type ATPase(P-ATPase) is involved in the formation of appressorium. A number of small molecular substances are able to enter the pathogen from the host during the interactions between pathogens and hosts, thus resisting the infection of pathogens. In this study, a 232 bp DNA sequence with good specificity from the first exon of P-ATPase gene MgAPT2 was used as an interference fragment and was inserted into interference vector forward and reversely.The interfering vector was then transformed into rice blast-susceptible rice variety Nipponbare via Agrobacterium-mediated transformation. Identification of rice plants inoculated with M. grisea at the seedling stage and detection of the expression level of P-ATPase gene MgAPT2 showed that the expression level of MgAPT2 gene in transgenic plants was reduced and the rice blast resistance was improved. This study provided a new way for the innovation of rice germplasm resources resistant to rice blast disease. [ABSTRACT FROM AUTHOR]