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Blueberries have high nutritional and economic value and are easy to cultivate, so they are common fruit crops in China. There is a high demand for blueberry in domestic and foreign markets, and various technologies have been used to extend the supply cycle of blueberry to about 7 months. However, blueberry grows in clusters, and a cluster of fruits generally contains fruits of different degrees of maturity, which leads to low efficiency in manually picking mature fruits, and at the same time wastes a lot of manpower and material resources. Therefore, in order to improve picking efficiency, it is necessary to adopt an automated harvesting mode. However, an accurate maturity detection model can provide a prerequisite for automated harvesting technology. Therefore, this paper proposes a blueberry ripeness detection model based on enhanced detail feature and content-aware reassembly. First of all, this paper designs an EDFM (Enhanced Detail Feature Module) that improves the ability of detail feature extraction so that the model focuses on important features such as blueberry color and texture, which improves the model's ability to extract blueberry features. Second, by adding the RFB (Receptive Field Block) module to the model, the lack of the model in terms of receptive field can be improved, and the calculation amount of the model can be reduced at the same time. Then, by using the Space-to-depth operation to redesign the MP (MaxPool) module, a new MP-S (MaxPool–Space to depth) module is obtained, which can effectively learn more feature information. Finally, an efficient upsampling method, the CARAFE (Content-Aware Reassembly of Features) module, is used, which can aggregate contextual information within a larger receptive field to improve the detection performance of the model. In order to verify the effectiveness of the method proposed in this paper, experiments were carried out on the self-made dataset "Blueberry—Five Datasets" which consists of data on five different maturity levels of blueberry with a total of 10,000 images. Experimental results show that the mAP (mean average precision) of the proposed network reaches 80.7%, which is 3.2% higher than that of the original network, and has better performance than other existing target detection network models. The proposed model can meet the needs of automatic blueberry picking. [ABSTRACT FROM AUTHOR]