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The main nitrogen (N) sources in soybean production originate from soil fixing bacteria Bradyrhizobium spp. and from mineralization of soil organic N. These sources of N are often not su cient to cover the N needs of the soybean. The present two-year field study aimed to evaluate the e ects of soybean genotypes (Valjevka and Galina) and rates of starter fertilizer N (0, 30, 60, and 90 kg ha�����1) on quantitative and qualitative parameters and on rain use e ciency (RUE) under contrasting weather conditions in the Pannonian region of Serbia. A field study conducted during two di erent growing seasons: first year with unfavorable weather conditions and second year with favorable weather conditions. As expected, the quantitative parameters, oil content, and RUE were higher in the year with favorable growing season, the second one. According to measured parameters, the genotype Valjevka performed higher yield potential as compared to the genotype Galina. The highest values of quantitative parameters and RUE were recorded at 60 kg N ha�����1, protein content at 90 kg N ha�����1 and oil content 0 kg N ha�����1 (control). This study suggests that proper genotype selection and application of 60 kg N ha�����1 as a starter dose with rhizobial inoculation could contribute to the high yield, while protein could be altered by N amount, independently on genotype.