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The European Commission's "Farm to Folk" strategy recommends reducing fertilizers by at least 20% by 2030. In this context, the aim of this study was to verify whether a 20 and 40% reduction of nitrogen (N) fertilization rate will be sufficient to maintain soil chemical features, fertility, and yields in monoculture maize cultivation in the no-tillage (NT) system versus the traditional plowing (PL) system. We also examined which tillage system (PL, NT) allows the reduction of fertilization while maintaining good yields of the tested soils. Two fields (10 ha each) were established for PL and NT maize cultivation, and soils (0–20 cm) were sampled twice per year—before maize sowing and after maize harvesting. A broad range of chemical and biological parameters were monitored (i.e., pH, forms of nitrogen, phosphorus and carbon, content of selected macronutrients and humic substances, and respiration activity). It was concluded that the 20% reduction in N fertilization (after 4 years of use) did not have an adverse effect on the soil's chemical and biological features, which mainly depended on the season of the year. The maize yield seemed to be higher in the PL system, which was mostly the result of the tillage system rather than the N dose. The study will be continued in the next vegetation season to further verify our findings, especially with regard to the maize tillage system and yields. [ABSTRACT FROM AUTHOR]

The aim of the study was to determine the Actinobacteria structure in cultivated (C) versus non-cultivated (NC) soils divided into three groups (autogenic, hydrogenic, lithogenic) with consideration its formation process in order to assess the Actinobacteria sensitivity to agricultural soil use and soil genesis and to identify factors affecting their abundance. Sixteen C soil samples and sixteen NC samples serving as controls were taken for the study. Next generation sequencing (NGS) of the 16S rRNA metagenomic amplicons (Ion Torrent™ technology) and Denaturing Gradient Gel Electrophoresis (DGGE) were applied for precise determination of biodiversity. Generally, greater abundance of Actinobacteria in the NC soils relative to the C soils was found. Moreover, it was indicated that the actinobacterial diversity depended on both the soil genesis and the land use; however, this effect directly depended on the particular family and genera. Two factors: redox potential (Eh) and total carbon (TC) seemed to had a significant effect on the diversity of Actinobacteria. More precisely, Actinobacteria from the NC soils displayed a greater affinity for each other and were clearly influenced by Eh, whilst those from the C soils were mostly influenced by TC. [ABSTRACT FROM AUTHOR]