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The timing of nitrogen fertilizer application in vineyards can determine the amount of nitrogen (N) absorbed, distributed, and accumulated in grapevine organs. The study aimed to evaluate the annual contribution of N from urea, applied at different times in Cabernet Sauvignon, grown in sandy and clayey soil in a subtropical climate. The sandy soil received 21.42 kg N ha−1 and the clayey soil 30 kg N ha−1, both enriched with 3% excess 15N atoms, applied at different times. The N derived from the fertilizer in grapevines, at all times of N application, and in sandy and clayey soil did not exceed 8%, with the highest values being observed in annual organs, especially in the leaves. The application of N marked at the phenological stages of IBB (50% at beginning of budbreak + 50% at full budbreak) enabled greater absorption of N derived from the fertilizer by the vines grown in both sandy and loamy soil. The N present in the annual organs (leaves, berries, stalks, and shoots) and in the perennial organs (stems and canes) of Cabernet Sauvignon grown in sandy and clayey soil was derived in greater percentages from the soil. [ABSTRACT FROM AUTHOR]