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A copper nanoparticle exhibit electromagnetic spectrum that is potential in a biosensor development. It is crucial to design a low cost nanoparticle biosensor that is easily fabricated at precise sizes and density. This paper provides a convenient method for copper nanoparticle deposition on a glass substrate with a magnetron sputtering process known as Physical Vapor Deposition. The main objective is to determine a reliable prediction recipe for various nanometer copper film thicknesses. In this experiment, six glass slides were coated with copper at different sputtering time. The time was varied from 280 sec to 980 sec while Argon gas and DC power were maintained respectively at 80 sccm and 130 watt. Later, the optics based measurement and electron microscope were employed for assessing the copper film thickness. The experiment result indicates different thicknesses were achieved from 35 nm to 45 nm at various sputtering time. A uniform and continuous coating were achieved as magnified by Scanning Electron Microscope. The result is beneficial for evaluating the anisotropy of the copper coating for a nanoscale microbial detection at different spectral wavelength. [ABSTRACT FROM AUTHOR]

Biosensor is one of the analytical devices that can detect chemical substance which contain biological component with a physicochemical detector. Optical biosensors are powerful alternative to conventional analytical techniques. Plasmonic biosensors are relying under optical biosensor categories. Previously, biosensor are normally use the tags and label-based detection and it will condition unbinding antigen towards the antibody such an example label is conjugated with one of the bio reactants, labeling can alter the biding properties. Plasmonic biosensor is a biosensor that uses Surface Plasmon Resonance (SPR) theory for its operation. Normally this biosensor is contains from a few components such as light source, prism, metal film, and detector. The metal film part is going to be discussed in this study where the SPR phenomenon will experienced. Gold nanoparticle is being used as the main material to fabricate the substrate and forming the thin film. Stated also gold have good chemical and mechanical stability which will affected the immobilization activity. For the exciting method use for surface plasmon, Krestchmann Configuration is being the most selected one. This obviously will produce a different kind of light reflection and dielectric properties. For the development of this plasmonic thin film the method is going to use is DC magnetron sputtering method and will be test on the rotational angle stage in order to get the optimum energy absorption that produce dark band as the intensity of light being absorb at certain angle. On the same time, simulation for SPR phenomenon oh this thin film was develop and resulting on produce graphical result on light refractive index [ABSTRACT FROM AUTHOR]