1. The effects of UV light wavelength and distance differences in viral genetic material integrity.
- Author
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Adiningrat, Arya, Fadhlurrahman, Ahmad Ghitha, Rismawanto, Yuli, Hariadi, Tony Khristanto, Ulfa, Maria, Wiyagi, Rama Okta, and Mustar, Muhammad Yusvin
- Subjects
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GENE amplification , *HEALTH facilities , *WAVELENGTHS , *MEDICAL equipment , *SARS-CoV-2 , *DISTANCES - Abstract
The pandemic has shown viral capacity in massive spreading and environmental adaptability for years. It has infected millions of people with several symptoms leading to millions of mortality worldwide. Its fast-spreading capacity, unpredictability prognosis and variants appearance are leading big threats, particularly to the health facilities. Therefore, appropriate health protection equipment, including adequate and safe decontamination procedures, is required to support the facilities in overcoming potential future contaminating pathogens. Unfortunately, only a few studies evaluated the utilization of medical decontamination devices. Rapid and massive manufacturing of decontamination devices without further and adequate post-monitoring may have several side effects. Therefore, careful and continuing consideration and evaluation studies are necessary to maintain the devices' quality and performance. This study aims to evaluate the ability of UV lights as laboratory devices to impair viral components. Clinical RNA samples were provided by the COVID-19 laboratory examiner as blinded samples without any subject information. SARS-CoV2 RNA (a viral representative RNA material) extract was exposed to 5 different UV lights, with different distances and exposure duration. The exposed RNA was then sequentially used as a cDNA template and DNA amplification. Amplification products were visualized by electrophoresis and densitometry analysis. Visualization and densitometry analysis showed that the UV-LED Nichia NCSU 334 had the most significant disruptive ability among the tested lights. The exposure duration and distance altered the effectiveness of UV light in damaging viral genetic material. UV-LED Nichia NCSU 334 could be suggested as an alternative UV source in a UV light-based disinfection system. This study proposed direct evidence for evaluating decontamination devices to ensure the effectiveness of the decontamination process. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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