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Non particle nano Ag conductive ink is a promising material for the patterning of flexible circuits by using the electrohydrodynamic micro printing technology. The pulse voltage control mold has been mainly used in the on demand droplet control in the patterning process. In this paper, the effect of pulse voltage amplitude on cone jetting behavior of this ink in one pulse period has been investigated by using the sequential images technology. Results indicated that under the same base direct current voltage applied on the nozzle, the pulse voltage applied on the substrate induced pulsant cone jetting rather than stable cone jetting in a pulse period if the value is under a critical value. When the voltage amplitude above this critical value was used, the stable cone jetting was obtained for each ejection. The increasing voltage amplitude resulted in the shortening of jetting starting time and also the ending time, and the jetting duration was decreased with the increasing of pulse voltage amplitude. Therefore, the jetting volume can be adjusted by adjusting the applied voltage amplitude. In addition, the profile evolution of the meniscus as the effect of pulse voltage has also been analysed by using a special designed analysis soft ware. [ABSTRACT FROM AUTHOR]