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Background: One of the most common cockroach types in urban areas, the American cockroach (Periplaneta americana), has been reported to impose an increased risk of allergies and asthma. Limited groups of allergens (Per a 1–13) have been identified in this species due to the lack of genome‐related information. Methods: To expand the allergen profile of P. americana, genomic, transcriptomic, and proteomic approaches were applied. With the support of a high‐quality genome assembled using nanopore, Illumina, and Hi‐C sequencing techniques, potential allergens were identified based on protein homology. Then, using enzyme‐linked immunosorbent assay, selected allergens were tested in Thai patients allergic to P. americana. Results: A chromosomal‐level genome of P. americana (3.06 Gb) has been assembled with 94.6% BUSCO completeness, and its contiguity has been significantly improved (N50 = 151 Mb). A comprehensive allergen profile has been characterized, with seven novel groups of allergens, including enolase (Per a 14), cytochrome C (Per a 15), cofilin (Per a 16), alpha‐tubulin (Per a 17), cyclophilin (Per a 18), porin3 (Per a 19), and peroxiredoxin‐6 (Per a 20), showing IgE sensitivity in enzyme‐linked immunosorbent assay. A new isoallergen of tropomyosin (Per a 7.02) and multiple potential isoallergens of Per a 5 were revealed using bioinformatics and proteomic approaches. Additionally, comparative analysis of P. americana with the closely related Blattodea species revealed the possibility of cross‐reaction. Conclusion: The high‐quality genome and proteome of P. americana are beneficial in studying cockroach allergens at the molecular level. Seven novel allergen groups and one isoallergen in Per a 7 were identified. [ABSTRACT FROM AUTHOR]

To the Editor, House dust mite (HDM) is one of the major allergen sources worldwide which causes up to 85% of respiratory allergic diseases.[1] This species was regarded as the most prevalent source of indoor allergens in the United States and Europe. In an attempt to study the stability of mite miRNAs in the environment, we have performed a Taqman miRNA expression assay (Applied Biosystems) to test the quantity of mite-specific miRNA in household dust samples. This is the first systemic investigation of dust mite miRNAs and a comprehensive picture of miRNAs in DPT was shown. [Extracted from the article]