1. Sequential class switch recombination to IgE and allergen-induced accumulation of IgE
- Author
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Almudena, Testera-Montes, Francisca, Palomares, Raquel, Jurado-Escobar, Ruben, Fernandez-Santamaria, Adriana, Ariza, Jesus, Verge, Maria, Salas, Paloma, Campo, Cristobalina, Mayorga, Maria Jose, Torres, Carmen, Rondon, and Ibon, Eguiluz-Gracia
- Subjects
Nasal Mucosa ,Nasal Provocation Tests ,Immunoglobulin G ,Humans ,Antigens, Dermatophagoides ,Allergens ,Immunoglobulin E ,Rhinitis, Allergic - Abstract
The involvement of allergen-specific (s)IgE in local allergic rhinitis (LAR) has been debated. Here, we investigate the effect of nasal allergen challenge with Dermatophagoides pteronyssinus (NAC-DP) in mucosal and peripheral B-cell subpopulations in LAR patients.Nine LAR, 5 allergic rhinitis (AR), and 5 non-atopic healthy control (HC) individuals were subjected to a 3-day NAC-DP protocol, and nasal biopsies and blood samples were collected before and after provocation. Nasal biopsies were used for immunohistochemistry and gene expression studies, whereas the frequency of lymphocyte subsets and basophil activation test (BAT) were analyzed in blood samples by flow cytometry. sIgG was measured in sera.NAC-DP induced an increase in IgEThese results suggest that allergen exposure induces the sequential εCSR of IgG
- Published
- 2022