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3 results on '"Mycobacterium massiliense"'

1. Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense

Author

Dongxin Liu, Wencong He, Mingxia Jiang, Bing Zhao, Xichao Ou, Chunfa Liu, Hui Xia, Yang Zhou, Shengfen Wang, Yuanyuan Song, Yang Zheng, Qian Chen, Jiale Fan, Guangxue He, and Yanlin Zhao

Subjects
Mycobacterium abscessus, Mycobacterium massiliense, Drug susceptibility testing, LAMP, erm(41) gene, Diagnosis, Biotechnology, TP248.13-248.65, Microbiology, QR1-502
Abstract

Abstract Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-resistance and cure rate. In the present study, a loop-mediated isothermal amplification (LAMP) coupled with lateral flow dipstick (LFD) method was developed to simultaneous detect M. abscessus and M. massiliense, via specific erm(41) gene. The amplification was carried out at 65 °C for only 60 min, and the results could be visualized on a lateral flow strip. Positive results only occurred in M. abscessus and M. massiliense, no cross-reaction with other mycobacterial species was observed. Therefore, the cost-effective MABC (M. abscessus complex)–LAMP–LFD method developed here was able to correct the diagnose of M. abscessus and M. massiliense infection in a short time. Thus, this method could be used to guide clinicians in treatment of M. abscessus group infections.

Published
2019
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2. Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense

Author

Yanlin Zhao, Jiale Fan, Qian Chen, Yang Zheng, Yuanyuan Song, Chunfa Liu, Guangxue He, Wencong He, Dongxin Liu, Mingxia Jiang, Xichao Ou, Bing Zhao, Shengfen Wang, Yang Zhou, and Hui Xia

Subjects
0106 biological sciences, Cure rate, lcsh:Biotechnology, Biophysics, Loop-mediated isothermal amplification, lcsh:QR1-502, Pulmonary disease, Mycobacterium abscessus, 01 natural sciences, Applied Microbiology and Biotechnology, lcsh:Microbiology, Microbiology, 03 medical and health sciences, LAMP, 010608 biotechnology, erm(41) gene, lcsh:TP248.13-248.65, Diagnosis, Medicine, Mycobacterium massiliense, 030304 developmental biology, 0303 health sciences, biology, business.industry, Dipstick, biology.organism_classification, bacterial infections and mycoses, Wound infection, bacteria, Original Article, business, Drug susceptibility testing
Abstract

Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-resistance and cure rate. In the present study, a loop-mediated isothermal amplification (LAMP) coupled with lateral flow dipstick (LFD) method was developed to simultaneous detect M. abscessus and M. massiliense, via specific erm(41) gene. The amplification was carried out at 65 °C for only 60 min, and the results could be visualized on a lateral flow strip. Positive results only occurred in M. abscessus and M. massiliense, no cross-reaction with other mycobacterial species was observed. Therefore, the cost-effective MABC (M. abscessus complex)–LAMP–LFD method developed here was able to correct the diagnose of M. abscessus and M. massiliense infection in a short time. Thus, this method could be used to guide clinicians in treatment of M. abscessus group infections. Electronic supplementary material The online version of this article (10.1186/s13568-019-0734-4) contains supplementary material, which is available to authorized users.

Published
2019

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