Search

Your search keyword '"Ambrosio, Maria Raffaella"' showing total 6 results
Start Over Author "Ambrosio, Maria Raffaella" Journal american journal of clinical pathology
6 results on '"Ambrosio, Maria Raffaella"'

1. Preferential Usage of Specific Immunoglobulin Heavy Chain Variable Region Genes With Unmutated Profile and Advanced Stage at Presentation Are Common Features in Patients With Chronic Lymphocytic Leukemia From Senegal

Author

Amato, Teresa, Sall, Abibatou, Dièye, Tandakha NDiaye, Gozzetti, Alessandro, Iacono, Michele, Ambrosio, Maria Raffaella, Granai, Massimo, Somma, Serena, Diop, Saliou, Touré, Awa Oumar, May, Evelyne, Gattiollat, Charles Henry, Wiels, Joëlle, Ahmed, Yonis, Raphael, Martine, Leoncini, Lorenzo, Bellan, Cristiana, and Piccaluga, Pier Paolo

Published
2017
Full Text
View/download PDF

3. Clonality Analysis of Immunoglobulin Gene Rearrangement by Next-Generation Sequencing in Endemic Burkitt Lymphoma Suggests Antigen Drive Activation of BCR as Opposed to Sporadic Burkitt Lymphoma

Author

Amato, Teresa, primary, Abate, Francesco, additional, Piccaluga, Pierpaolo, additional, Iacono, Michele, additional, Fallerini, Chiara, additional, Renieri, Alessandra, additional, De Falco, Giulia, additional, Ambrosio, Maria Raffaella, additional, Mourmouras, Vaselious, additional, Ogwang, Martin, additional, Calbi, Valeria, additional, Rabadan, Roul, additional, Hummel, Michael, additional, Pileri, Stefano, additional, Leoncini, Lorenzo, additional, and Bellan, Cristiana, additional

Published
2015
Full Text
View/download PDF

5. Clonality Analysis of Immunoglobulin Gene Rearrangement by Next-Generation Sequencing in Endemic Burkitt Lymphoma Suggests Antigen Drive Activation of BCR as Opposed to Sporadic Burkitt Lymphoma.

Author

Amato, Teresa, Abate, Francesco, Piccaluga, Pierpaolo, Iacono, Michele, Fallerini, Chiara, Renieri, Alessandra, De Falco, Giulia, Ambrosio, Maria Raffaella, Mourmouras, Vaselious, Ogwang, Martin, Calbi, Valeria, Rabadan, Roul, Hummel, Michael, Pileri, Stefano, Leoncini, Lorenzo, and Bellan, Cristiana

Subjects
BURKITT'S lymphoma, TRANSCRIPTION factors, HELIX-loop-helix motifs, GENETIC mutation, RNA sequencing, B cell receptors, IMMUNOGLOBULINS, B cell lymphoma, GENES, PROTEINS, SEQUENCE analysis
Abstract

Objectives: Recent studies using next-generation sequencing (NGS) analysis disclosed the importance of the intrinsic activation of the B-cell receptor (BCR) pathway in the pathogenesis of sporadic Burkitt lymphoma (sBL) due to mutations of TCF3/ID3 genes. Since no definitive data are available on the genetic landscape of endemic Burkitt (eBL), we first assessed the mutation frequency of TCF3/ID3 in eBL compared with sBL and subsequently the somatic hypermutation status of the BCR to answer whether an extrinsic activation of BCR signaling could also be demonstrated in Burkitt lymphoma.Methods: We assessed the mutations of TCF3/ID3 by RNAseq and the BCR status by NGS analysis of the immunoglobulin genes (IGs).Results: We detected mutations of TCF3/ID3 in about 30% of the eBL cases. This rate is significantly lower than that detected in sBL (64%). The NGS analysis of IGs revealed intraclonal diversity, suggesting an active targeted somatic hypermutation process in eBL compared with sBL.Conclusions: These findings support the view that the antigenic pressure plays a key role in the pathogenetic pathways of eBL, which may be partially distinct from those driving sBL development. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

6. Preferential Usage of Specific Immunoglobulin Heavy Chain Variable Region Genes With Unmutated Profile and Advanced Stage at Presentation Are Common Features in Patients With Chronic Lymphocytic Leukemia From Senegal

Author

Joëlle Wiels, Martine Raphael, Serena Somma, Cristiana Bellan, Alessandro Gozzetti, Massimo Granai, Evelyne May, Awa Oumar Touré, Lorenzo Leoncini, Yonis Ahmed, Michele Iacono, Tandakha Ndiaye Dieye, Charles Henry Gattiollat, Pier Paolo Piccaluga, Abibatou Sall, Teresa Amato, Saliou Diop, Maria Raffaella Ambrosio, Amato, Teresa, Sall, Abibatou, Dièye, Tandakha Ndiaye, Gozzetti, Alessandro, Iacono, Michele, Ambrosio, Maria Raffaella, Granai, Massimo, Somma, Serena, Diop, Saliou, Touré, Awa Oumar, May, Evelyne, Gattiollat, Charles Henry, Wiels, Joëlle, Ahmed, Yoni, Raphael, Martine, Leoncini, Lorenzo, Bellan, Cristiana, and Piccaluga, Pier Paolo

Subjects
Male, Chronic lymphocytic leukemia, Immunoglobulin heavy chain genes rearrangements, Immunoglobulin heavy chain variable region genes, 2734, Immunoglobulin Heavy Chain, Genes, Immunoglobulin Heavy Chain, Immunoglobulin Variable Region, Immunoglobulin heavy chain genes rearrangement, Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, hemic and lymphatic diseases, medicine, Tumor Microenvironment, Humans, In patient, Amino Acid Sequence, Immunoglobulin heavy chain variable region gene, Gene, business.industry, Repertoire, Advanced stage, General Medicine, medicine.disease, Leukemia, Lymphocytic, Chronic, B-Cell, Senegal, Leukemia, 030220 oncology & carcinogenesis, Multigene Family, Immunology, Cohort, Immunoglobulin heavy chain, Female, Cohort Studie, business, Immunoglobulin Heavy Chains, 030215 immunology, Human
Abstract

Objectives: Chronic lymphocytic leukemia (CLL) is the most common type of leukemia in Western populations, being rarer in Asian and African people. It has been suggested that patients with CLL from Africa might have a more aggressive disease compared with white patients. In this study, we aimed to identify genetic factors that may account for this difference. Methods: We analyzed immunoglobulin heavy chain (IGH) genes' mutational status by performing next-generation sequencing in 25 Senegalese and 50 Italian patients with CLL. Results: We found that Senegalese patients more frequently had adverse prognostic factors and an unmutated profile. Furthermore, we documented that IGHV1 (IGHV1-69), IGHD3, and IGHJ6 were significantly more frequent in Senegalese patients, whereas IGHV3-30 was common and limited to the Italian cohort. Stereotyped receptors commonly detected in the white population were not recorded in our Senegalese series. Conclusions: The different IGH repertoire we observed in the Senegalese cohort may reflect the diverse genetic and microenvironmental (ie, polymicrobial stimulation) background.

Published
2017

Catalog

Books, media, physical & digital resources
See catalog results