1. Optimization of Pancreatic Juice Collection: A First Step Toward Biomarker Discovery and Early Detection of Pancreatic Cancer.
- Author
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Levink, Iris J. M., Nesteruk, Kateryna, Visser, Dido I., Sieuwerts, Anieta M., Fernandes, Celio J. C., Jansen, Maurice P. H. M., van Driel, Lydi M. J. W., Poley, Jan-Werner, Peppelenbosch, Maikel P., Cahen, Djuna L., Fuhler, Gwenny M., and Bruno, Marco J.
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SURVEILLANCE detection , *ADENOCARCINOMA , *BIOMARKERS , *PANCREATIC secretions , *ORGANOIDS - Abstract
INTRODUCTION: Imaging-based surveillance programs fail to detect pancreatic ductal adenocarcinoma at a curable stage, creating an urgent need for diagnostic biomarkers. METHODS: Secretin-stimulated pancreatic juice (PJ) was collected from the duodenal lumen during endoscopic ultrasound. The yield of biomarkers and organoids was compared for 2 collection techniques (endoscope suction channel vs catheter-based) and 3 periods (0-4 vs 4-8 vs 8-15 minutes). RESULTS: Collection through the endoscope suction channel was superior to collection with a catheter. Collection beyond 8 minutes reduced biomarker yield. PJ-derived organoid culture was feasible. DISCUSSION: The optimal protocol for secretin-stimulated PJ collection is through the endoscope suction channel for 8 minutes allowing biomarker detection and organoid culture. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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