1. A de novo philadelphia chromosome-positive acute mixed-lineage leukemia with both major and minor BCR/ABL mRNA transcripts
- Author
-
T, Tarumoto, S, Imagawa, K, Ohmine, A, Mano, T, Nagai, M, Takatoku, K, Muroi, K, Hatake, and K, Ozawa
- Subjects
Adult ,Gene Rearrangement ,Male ,Histocytochemistry ,Reverse Transcriptase Polymerase Chain Reaction ,Remission Induction ,Fusion Proteins, bcr-abl ,Bone Marrow Cells ,Burkitt Lymphoma ,Translocation, Genetic ,Blotting, Southern ,Leukemia, Myeloid, Acute ,Antineoplastic Combined Chemotherapy Protocols ,Humans ,Philadelphia Chromosome ,RNA, Messenger ,Immunoglobulin Heavy Chains ,Peroxidase - Abstract
A patient with a Philadelphia chromosome (Ph)-positive acute mixed-lineage leukemia (AMLL) expressing both major and minor BCR/ABL mRNA transcripts is described. Phenotypic analysis of the leukemic blasts revealed positivity for both myeloid and B-cell lineages. Southern blot analysis showed a rearrangement of the immunoglobulin heavy chain (IgH) gene. Reverse transcriptase polymerase chain reaction (RT-PCR) analysis showed the expression of both major and minor BCR/ABL mRNA transcripts. To our knowledge, this is the first report of AMLL expressing both major and minor BCR/ABL mRNA transcripts and rearrangement of the IgH gene.
- Published
- 2000