1. Rapid chromosome detection by PRINS in human sperm
- Author
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Isabelle Imbert, Brigitte Andréo, and Franck Pellestor
- Subjects
Genetics ,Autosome ,Double labeling ,Chromosome ,Aneuploidy ,Biology ,medicine.disease ,Sperm ,Andrology ,Nondisjunction ,medicine ,Primer (molecular biology) ,Chromosome 21 ,Genetics (clinical) - Abstract
Primed in situ (PRINS) labeling may be used for direct estimation of disomy rate in human sperm. We combined the PRINS procedure with a rapid and efficient NaOH pretreatment allowing simultaneous decondensation and denaturation of sperm nuclei. This enabled double labeling of human sperm within a 2-hr time span. Chromosome-specific primers have been defined for most human chromosomes, including those most frequently involved in aneuploidy. Using the dual-color PRINS method, we estimated incidences of disomy for 15 autosomes and the sex chromosomes in sperm samples from 6 normal fertile men. The frequency of disomy ranged from 0.28% to 0.36%. There were no significant interchromosomal differences in disomy rate, but chromosome 21 displayed a higher incidence of nondisjunction than did the other chromosomes.
- Published
- 2001