1. Effect of low-frequency mechanical vibration on orthodontic tooth movement.
- Author
-
Yadav S, Dobie T, Assefnia A, Gupta H, Kalajzic Z, and Nanda R
- Subjects
- Acid Phosphatase analysis, Adaptor Proteins, Signal Transducing, Alveolar Process cytology, Alveolar Process physiology, Animals, Azo Compounds, Biomechanical Phenomena, Bone Morphogenetic Proteins antagonists & inhibitors, Bone Remodeling physiology, Collagen analysis, Coloring Agents, Dental Alloys chemistry, Glycoproteins analysis, Intercellular Signaling Peptides and Proteins, Isoenzymes analysis, Male, Mice, Nickel chemistry, Orthodontic Wires, Osteoclasts cytology, Periodontal Ligament physiology, Random Allocation, Tartrate-Resistant Acid Phosphatase, Titanium chemistry, Tooth Movement Techniques instrumentation, X-Ray Microtomography methods, Molar physiology, Tooth Movement Techniques methods, Vibration
- Abstract
Introduction: Our objective was to investigate the effect of low-frequency mechanical vibration (LFMV) on the rate of tooth movement, bone volume fraction, tissue density, and the integrity of the periodontal ligament. Our null hypothesis was that there would be no difference in the amount of tooth movement between different values of LFMV., Methods: Sixty-four male CD1 mice, 12 weeks old, were used for orthodontic tooth movement. The mice were randomly divided into 2 groups: control groups (baseline; no spring + 5 Hz; no spring + 10 Hz; and no spring + 20 Hz) and experimental groups (spring + no vibration; spring + 5 Hz; spring + 10 Hz; and spring + 20 Hz). In the experimental groups, the first molars were moved mesially for 2 weeks using nickel-titanium coil springs delivering 10 g of force. In the control and experimental groups, LFMV was applied at 5, 10, or 20 Hz. Microfocus x-ray computed tomography analysis was used for tooth movement measurements, bone volume fraction, and tissue density. Additionally, immunostaining for sclerostin, tartrate-resistant acid phosphatase (TRAP) staining, and picrosirius red staining were used on the histologic sections. Simple descriptive statistics were used to summarize the data. Kruskal-Wallis tests were used to compare the outcomes across treatment groups., Results: LFMV did not increase the rate of orthodontic tooth movement. Microfocus x-ray computed tomography analysis showed increases in bone volume fractions and tissue densities with applications of LFMV. Sclerostin expression was decreased with 10 and 20 Hz vibrations in both the control and experimental groups. Additionally, the picrosirius staining showed that LFMV helped in maintaining the thickness and integrity of collagen fibers in the periodontal ligament., Conclusions: There was no significant increase in tooth movement by applying LFMV when compared with the control groups (spring + no vibration)., (Copyright © 2015 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF