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Reports that AE2 messenger RNA is detectable throughout the nephron, with maximal levels in the medullary thick ascending limb, approximately twice the level in the proximal tubule. Compatibility with the postulated roles of AE2 in the maintenance of intracellular pH and chloride concentration and with its possible participation in transepithelial transport.

Examines hypoxia-mediated cell death pathways in Jurkat cells in the presence and absence of extracellular glucose. Effect of hypoxia on cytochrome c release, polymerase cleavage and DNA fragmentation; Impact of the overexpression of caspase 8 v-FLIP inhibitor on hypoxia-mediated cell; Activation of death receptor pathway.

Tests the hypothesis that antisense GLUT-1 protects mesangial cells from glucose induction of GLUT-1 and fibronectin (FN) expression. Immunoprecipitation of FN protein to measure changes in its synthesis by mesangial cells; Development of a clone of antisense GLUT-1 transduced mesangial cells.