1. High-temporal-resolution analysis demonstrates that ICAM-1 stabilizes WEHI 274.1 monocytic cell rolling on endothelium.
- Author
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Kevil CG, Chidlow JH, Bullard DC, and Kucik DF
- Subjects
- Animals, Antineoplastic Agents pharmacology, Aorta cytology, Cell Adhesion immunology, Cell Communication immunology, Cells, Cultured, Endothelium, Vascular drug effects, Intercellular Adhesion Molecule-1 genetics, Mice, Mice, Mutant Strains, Microscopy, Video, P-Selectin metabolism, Tumor Necrosis Factor-alpha pharmacology, Endothelium, Vascular cytology, Endothelium, Vascular metabolism, Intercellular Adhesion Molecule-1 metabolism, Monocytes cytology, Monocytes metabolism
- Abstract
Leukocyte rolling, adhesion, and migration on vascular endothelium involve several sets of adhesion molecules that interact simultaneously. Each of these receptor-ligand pairs may play multiple roles. We examined the role of ICAM-1 in adhesive interactions with mouse aortic endothelial cells (MAECs) in an in vitro flow system. Average rolling velocity of the monocytic cell line WEHI 274.1 was increased on ICAM-1-deficient MAECs compared with wild-type MAECs, both with and without TNF-alpha stimulation. High-temporal-resolution analysis provided insights into the underlying basis for these differences. Without TNF-alpha stimulation, average rolling velocity was slower on wild-type than on ICAM-1-deficient endothelium because of brief (<1 s) pauses. On TNF-alpha-stimulated ICAM-1-deficient endothelium, cells rolled faster because of transient accelerations, producing "jerky" rolling. Firm adhesion to ICAM-1-deficient MAECs was significantly reduced compared with wild-type MAECs, although the number of rolling cells was similar. These results demonstrate directly that ICAM-1 affects rolling velocity by stabilizing leukocyte rolling.
- Published
- 2003
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