1. Comparative studies of the role of hormone-sensitive lipase and adipose triglyceride lipase in human fat cell lipolysis
- Author
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Dominique Langin, Mikael Rydén, Lennart Blomqvist, Peter Arner, Ellen E. Blaak, Johan Hoffstedt, Johan W. E. Jocken, Vanessa van Harmelen, Andrea Dicker, Mikael Wirén, Aline Mairal, Humane Biologie, RS: NUTRIM School of Nutrition and Translational Research in Metabolism, RS: NUTRIM - R1 - Metabolic Syndrome, Department of medicine [Stockholm], Karolinska Institutet [Stockholm]-Karolinska University Hospital [Stockholm], Department of Surgery, Laboratoire de Biochimie [CHU Toulouse], Institut Fédératif de Biologie (IFB), Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Department of Human Biology, Nutrition and Toxicology, Maastricht University [Maastricht], Unité de recherche sur les obésités, IFR 31 Louis Bugnard (IFR 31), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Simon, Marie Francoise
- Subjects
Glycerol ,Human fat ,Physiology ,Endocrinology, Diabetes and Metabolism ,Hormone-sensitive lipase ,MESH: Down-Regulation ,MESH: Lipase ,Cohort Studies ,chemistry.chemical_compound ,Norepinephrine ,Adipocyte ,Adipocytes ,MESH: Obesity ,MESH: Cohort Studies ,MESH: Sterol Esterase ,food and beverages ,Biochemistry ,MESH: Phospholipases A ,Female ,RNA Interference ,Polycystic Ovary Syndrome ,Adult ,medicine.medical_specialty ,Lipolysis ,MESH: RNA Interference ,Triacylglycerol lipase ,Down-Regulation ,Biology ,Phospholipases A ,MESH: Glycerol ,MESH: Norepinephrine ,Physiology (medical) ,Internal medicine ,medicine ,Humans ,MESH: Lipolysis ,Obesity ,Lipase ,MESH: Adipocytes ,MESH: Humans ,MESH: Adult ,Mesenchymal Stem Cells ,Sterol Esterase ,MESH: Mesenchymal Stem Cells ,Endocrinology ,chemistry ,Adipose triglyceride lipase ,biology.protein ,MESH: Polycystic Ovary Syndrome ,MESH: Female ,Hormone - Abstract
Hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL) regulate adipocyte lipolysis in rodents. Objective: To compare the roles of these lipases for lipolysis in human adipocytes. Design: Subcutaneous adipose tissue was investigated. HSL and ATGL protein expression were related to lipolysis in isolated mature fat cells. ATGL or HSL were knocked down by RNA interference or selectively inhibited and effects on lipolysis studied in differentiated preadipocytes or adipocytes derived from human mesenchymal stem cells (hMSC). Setting and subjects: Outpatient investigation. Subjects were all women, 12 lean controls, 8 lean with polycystic ovary syndrome and 27 otherwise healthy obese. Results: Noradrenaline-induced lipolysis was positively correlated with HSL protein levels (P
- Published
- 2007