1. Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow
- Author
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Steen Seier Poulsen, Elisa P. Jensen, Carolyn F. Deacon, Hannelouise Kissow, Niels-Henrik Holstein-Rathlou, Jens J. Holst, Boye L. Jensen, and Charlotte Mehlin Sorensen
- Subjects
Afferent arteriole ,Afferent arterioles ,Vascular smooth muscle ,Time Factors ,Physiology ,Peptide ,Blood Pressure ,Kidney ,Muscle, Smooth, Vascular ,Renin/blood ,Rats, Sprague-Dawley ,Glucagon-Like Peptide 1/metabolism ,Glucagon-Like Peptide 1 ,Heart Rate ,Renin ,Receptors, Glucagon ,Homeostasis ,Receptor ,Sodium/blood ,Peptides/pharmacology ,chemistry.chemical_classification ,Venoms/pharmacology ,Chemistry ,digestive, oral, and skin physiology ,Arterioles/metabolism ,Glucagon-like peptide-1 ,Immunohistochemistry ,Arterioles ,medicine.anatomical_structure ,Potassium/blood ,Female ,Myocytes, Smooth Muscle/drug effects ,hormones, hormone substitutes, and hormone antagonists ,Signal Transduction ,endocrine system ,medicine.medical_specialty ,Myocytes, Smooth Muscle ,Receptors, Glucagon/agonists ,Glucagon-Like Peptide-1 Receptor ,Renal Circulation ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Kidney/blood supply ,Venoms ,Microcirculation ,Sodium ,Renal Circulation/drug effects ,Mice, Inbred C57BL ,Renal Elimination ,Urodynamics ,Endocrinology ,Renal blood flow ,Potassium ,Autoradiography ,Exenatide ,Muscle, Smooth, Vascular/drug effects ,Peptides - Abstract
Glucagon-like peptide (GLP)-1 has a range of extrapancreatic effects, including renal effects. The mechanisms are poorly understood, but GLP-1 receptors have been identified in the kidney. However, the exact cellular localization of the renal receptors is poorly described. The aim of the present study was to localize renal GLP-1 receptors and describe GLP-1-mediated effects on the renal vasculature. We hypothesized that renal GLP-1 receptors are located in the renal microcirculation and that activation of these affects renal autoregulation and increases renal blood flow. In vivo autoradiography using 125I-labeled GLP-1, 125I-labeled exendin-4 (GLP-1 analog), and 125I-labeled exendin 9–39 (GLP-1 receptor antagonist) was performed in rodents to localize specific GLP-1 receptor binding. GLP-1-mediated effects on blood pressure, renal blood flow (RBF), heart rate, renin secretion, urinary flow rate, and Na+ and K+ excretion were investigated in anesthetized rats. Effects of GLP-1 on afferent arterioles were investigated in isolated mouse kidneys. Specific binding of 125I-labeled GLP-1, 125I-labeled exendin-4, and 125I-labeled exendin 9–39 was observed in the renal vasculature, including afferent arterioles. Infusion of GLP-1 increased blood pressure, RBF, and urinary flow rate significantly in rats. Heart rate and plasma renin concentrations were unchanged. Exendin 9–39 inhibited the increase in RBF. In isolated murine kidneys, GLP-1 and exendin-4 significantly reduced the autoregulatory response of afferent arterioles in response to stepwise increases in pressure. We conclude that GLP-1 receptors are located in the renal vasculature, including afferent arterioles. Activation of these receptors reduces the autoregulatory response of afferent arterioles to acute pressure increases and increases RBF in normotensive rats.
- Published
- 2014
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