1. Antiallergic Effect of Anti–Siglec-F through Reduction of Eosinophilic Inflammation in Murine Allergic Rhinitis
- Author
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Sung Hye Ahn, Young Hyo Kim, Dae Hyun Lim, Tae Young Jang, Byong Kwan Son, Kwangmin Na, Jeong Hee Kim, Chang-Shin Park, and Bruce S. Bochner
- Subjects
Rhinitis, Allergic, Perennial ,Antigens, Differentiation, Myelomonocytic ,Apoptosis ,Immunoglobulin E ,Group A ,Mice ,Antigen ,Anti-Allergic Agents ,Animals ,Immunology and Allergy ,Medicine ,Inflammation ,Sialic Acid Binding Immunoglobulin-like Lectins ,Mice, Inbred BALB C ,Lamina propria ,biology ,medicine.diagnostic_test ,business.industry ,General Medicine ,respiratory system ,Rhinitis, Allergic ,Eosinophils ,Disease Models, Animal ,Ovalbumin ,Treatment Outcome ,medicine.anatomical_structure ,Bronchoalveolar lavage ,Otorhinolaryngology ,Immunology ,biology.protein ,Nasal administration ,Antibody ,business - Abstract
Background Sialic acid-binding Ig-like lectin-F (Siglec-F) in mice and its functional paralog Siglec-8 in humans are transmembrane receptors that play a role in the apoptosis of eosinophils. We aimed to evaluate the therapeutic potential of anti-Siglec-F antibodies in a murine model of allergic rhinitis. Methods Twenty-eight BALB/c mice were used. In group A (control group, n = 7), mice were sensitized and challenged with saline. In group B (ovalbumin [OVA] challenge group, n = 7), OVA was used for i.p. sensitization and intranasal challenge. Mice in group C (control IgG group, n = 7) or those in group D (anti-Siglec-F group, n = 7) had been given rabbit control IgG or anti-Siglec-F antibody injections, respectively. We assessed the number of nose-scratching events; serum total/OVA-specific IgE; the number of eosinophils, neutrophils, and lymphocytes in bronchoalveolar lavage (BAL) fluid; histopathological changes in nasal cavity tissues; and the levels of IL-4, IL-5, and IL-13 in BAL fluid. Results Mice in group D had significantly less nose scratching. Serum total and OVA-specific IgE were not significantly changed. The number of eosinophils in BAL fluid and in the lamina propria of the nasal cavity mucosa was significantly decreased with anti-Siglec-F antibody treatment. The levels of Th2 cytokines such as IL-4, IL-5, and IL-13 were also significantly decreased with anti-Siglec-F antibody treatment. Conclusion Anti-Siglec-F antibody has beneficial effects in a mouse model of experimental allergic rhinitis.
- Published
- 2013
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