1. Gene Expression Profiling of Acute Liver Stress During Living Donor Liver Transplantation
- Author
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Jenny Heathcote, L. L. Tannis, David R. Grant, Jing Sun, Ivan Borozan, Aled M. Edwards, Ian D. McGilvray, O. D. Rotstein, Limin Chen, and Maha Guindi
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,Surgical stress ,medicine.medical_treatment ,Ischemia ,Gene Expression ,Liver transplantation ,Chronic liver disease ,Pathogenesis ,Andrology ,Living Donors ,medicine ,Humans ,Immunology and Allergy ,Pharmacology (medical) ,Oligonucleotide Array Sequence Analysis ,Transplantation ,business.industry ,Gene Expression Profiling ,Liver Diseases ,Middle Aged ,medicine.disease ,Liver Transplantation ,Gene expression profiling ,Genes ,Liver ,Reperfusion Injury ,Chronic Disease ,business ,Reperfusion injury - Abstract
During liver transplantation, the donor graft is subjected to a number of acute stresses whose molecular basis is not well-understood. The effects of surgical stress, preservation and reperfusion injury were studied in 24 consecutive living donor liver transplant (LDLT) operations. Liver biopsies were taken early in the donor operation (OPENING), after transection of the donor liver (PRECLAMP) and following implantation of the graft (post hepatic artery, [PHA]); these were evaluated for histology, tissue glutathione content and gene expression using a 19K-human cDNA microarray. LDLT was associated with an ischemia/reperfusion injury, with accumulation of small numbers of neutrophils and decreased glutathione in the PHA biopsies. Following reperfusion, the expression of 129 genes increased and 106 genes decreased when compared to OPENING levels (> or
- Published
- 2006
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