1. Expression of transglutaminase-2 isoforms in normal human tissues and cancer cell lines: dysregulation of alternative splicing in cancer.
- Author
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Phatak VM, Croft SM, Rameshaiah Setty SG, Scarpellini A, Hughes DC, Rees R, McArdle S, and Verderio EA
- Subjects
- Adenocarcinoma genetics, Aged, Amino Acid Sequence, Base Sequence, Cell Line, Tumor, GTP-Binding Proteins, Humans, Isoenzymes genetics, Isoenzymes metabolism, Male, Molecular Sequence Data, Prostatic Neoplasms genetics, Protein Glutamine gamma Glutamyltransferase 2, RNA Splice Sites, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Transglutaminases genetics, Adenocarcinoma enzymology, Alternative Splicing, Gene Expression, Prostatic Neoplasms enzymology, Transglutaminases metabolism
- Abstract
The multiple enzymatic activities and functions of transglutaminase type 2 (TG2) may be attributed to alternative TG2 molecules produced by differential splicing of TG2 mRNA. Different RNA transcripts of the human TG2 gene (TGM2) have been identified, but the expression of TG2 multiple transcripts has never been systematically addressed. We have confirmed and rationalized the main TG2 variants and developed a screening assay for the detection of alternative splicing of TG2, based on real-time reverse-transcription PCR. We have quantified the multiple TG2 transcripts in a wide range of normal tissues and in cancer cell lines from four different sites of origin. Our data show a significant correlation in the expression of canonical and alternative TG2 isoforms in normal human tissue, but differences in alternative splicing of TG2 in cancer cell lines, suggesting that in cancer cells the alternative splicing of TG2 is a more active process.
- Published
- 2013
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