1. Establishing a quantitative fluorescence assay for the rapid detection of kynurenine in urine.
- Author
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Patel, Kamlesh, Fernandez-Villamarin, Marcos, Ward, Craig, Lord, Janet M., Tino, Peter, and Mendes, Paula M.
- Subjects
KYNURENINE ,FLUORESCENCE ,URINE ,DETECTION limit ,STANDARD deviations - Abstract
The kynurenine metabolite is associated with many diseases and disorders, ranging from diabetes and sepsis to more recently COVID-19. Here we report a fluorescence-based assay for the detection of kynurenine in urine using a specific chemosensor, 3-formyl-4-(ethylthio)-7-(diethylamino)-coumarin. The assay produces a linear response at clinically relevant ranges (1–20 μM), with a limit of detection of 0.7 μM. The average standard addition recoveries of kynurenine in synthetic urine samples are near to 100%, and the relative standard deviation values are less than 8%. The established fluorescence assay for quantitative analysis of kynurenine in urine is facile, sensitive and accurate and holds great potential for low-cost and high-throughput analysis of kynurenine in clinical laboratory settings. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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