1. Fusion data from FT-IR and MALDI-TOF MS result in more accurate classification of specific microbiota.
- Author
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Gao, Wenjing, Han, Ying, Chen, Liangqiang, Tan, Xue, Liu, Jieyou, Xie, Jinghang, Li, Bin, Zhao, Huilin, Yu, Shaoning, Tu, Huabin, Feng, Bin, and Yang, Fan
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MULTISENSOR data fusion , *BACTERIA classification , *HIERARCHICAL clustering (Cluster analysis) , *RIBOSOMAL proteins , *CLUSTER analysis (Statistics) - Abstract
Microbes are usually present as a specific microbiota, and their classification remains a challenge. MALDI-TOF MS is particularly successful in library-based microbial identification at the species level as it analyzes the molecular weight of peptides and ribosomal proteins. FT-IR allows more accurate classification of bacteria at the subspecies level due to the high sensitivity, specificity and repeatability of FT-IR signals from bacteria, which is not achievable with MALDI-TOF MS. Previous studies have shown that more accurate identification results can be obtained by the fusion of FT-IR and MALDI-TOF MS spectral data. Here, we constructed 20 groups of model microbiota samples and used FT-IR, MALDI-TOF MS, and their fusion data to classify them. Hierarchical clustering analysis (HCA) showed that the classification accuracy of FT-IR, MALDI-TOF MS, and the fusion data was 85%, 90%, and 100%, respectively. These results indicate that both FT-IR and MALDI-TOF MS can effectively classify specific microbiota, and the fusion of their spectral data could improve the classification accuracy. The FT-IR and MALDI-TOF MS data fusion strategy may be a promising technology for specific microbiota classification. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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