1. Syrian hamster embryo (SHE) assay (pH 6.7) coupled with infrared spectroscopy and chemometrics towards toxicological assessment.
- Author
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Trevisan J, Angelov PP, Patel II, Najand GM, Cheung KT, Llabjani V, Pollock HM, Bruce SW, Pant K, Carmichael PL, Scott AD, and Martin FL
- Subjects
- Animals, Cell Transformation, Neoplastic drug effects, Cricetinae, Discriminant Analysis, Embryo, Mammalian cytology, Principal Component Analysis, Biological Assay instrumentation, Biological Assay methods, Embryo, Mammalian drug effects, Mesocricetus embryology, Organic Chemicals pharmacology, Spectroscopy, Fourier Transform Infrared instrumentation, Spectroscopy, Fourier Transform Infrared methods
- Abstract
The Syrian hamster embryo (SHE) assay (pH 6.7) is an in vitro candidate to replace in vivo carcinogenicity tests. However, the conventional method of visual scoring of foci (non-transformed vs. transformed colonies) can be time-consuming and is open to subjectivity. Infrared (IR) spectroscopy has the potential to provide objective assessment of such SHE colonies with the added advantage of potentially providing mechanistic information. In this study, SHE cells were treated with one of eight different chemical regimens, allowed in culture to attach and form foci on IR-reflective glass slides; these were subsequently interrogated by attenuated total reflection (ATR) Fourier-transform IR (FTIR) spectroscopy. Derived mid-IR spectra (n = 13,406) were subjected to chemometric analysis focusing primarily on the extraction of biochemical information related to test agent treatment and/or morphological transformation. The use of ATR-FTIR spectroscopy with chemometrics to analyze the SHE assay is a novel approach to toxicological assessment.
- Published
- 2010
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