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Herein, a cost-effective, simple and sensitive electrochemical sensing platform was established based on aptamer - target recognition and target-triggering signal amplification strategy for protein detection. Due to the high affinity between the aptamer and target, the assistant DNA1 (a1) could release from a1-aptamer duplex and trigger the following DNA circuits. The strand displacement and branch migration reaction brought assistant DNA3 (a3) released. Eventually, a large number of duplex structures of a3-Hairpin DNA3 were formed on the surface of electrode. Consequently, the capture DNA on the surface of platinum nanoparticles could hybridize with the unfolded DNA fragment of Hairpin DNA3 on the sensor surface, resulting in the electrochemical signal readout of H 2 O 2 reduction. Using thrombin as a model target, under the optimal conditions, this method exhibited a linear detection range from 0.5 pM to 300 nM with a detection limit of 0.17 pM. The proposed detection strategy was enzyme-free and exhibited good selectivity and sensitivity for a variety of protein targets detection by using corresponding DNA-based affinity probes, which makes it possible to apply the sensor for sensitivity detection of analytes in bioassays., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Here a sensing strategy with the integration of photosensitizer and electrochemical analysis was present. The photosensitizer, Zinc(II) tetraphenylporphyrin (ZnTCPP), was functionalized graphene oxide (GO) to form complex (ZnTCPP/GO) as the electrode material and generated singlet-oxygen ( 1 O 2 ) in the presence of air under light illumination. Due to the special electronic structure of 1 O 2 , hydroquinone (HQ) could react with 1 O 2 to produce electrochemically-detectable products, benzoquinone (BQ). Meanwhile, the formed BQ could be reduced on the electrode, completing the redox cycling. The ZnTCPP/GO modified ITO electrode produces a stable and enhanced photocurrent signal under 420 nm irradiation in air-saturated buffer, compared with in N 2 -saturated buffer. On the other hand, l-glutathione (GSH) as a signalling molecule plays important role in physiological process, which was employed as model to investigated the sensing performance. Coupling with HQ oxidized by 1 O 2 , a GSH sensor was constructed on the basis the redox cycling of HQ. A sensitive reduction of photocurrent is observed with the addition of GSH, due to the GSH could be oxidized by the generated 1 O 2 to form GSSG. The biosensor displayed good performance in a broad concentration range of 0-150 μM, with a lower detection limit of 1.3 μM at an S/N ratio of 3, and could be used in practical application. This work affords a platform for constructing the biosensor with 1 O 2 instead of enzyme via on/off light switching., Competing Interests: Declaration of competing interest We declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)